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SDU-Denmark/13 July 2010
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(New page: '''Phototaxis:'''<br><br> ''Progress report:'' We have attempted transformations with promoter, RBS and terminator for our assembly again. Apparently there might be a problem with our LA p...)
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Revision as of 19:09, 13 July 2010
Phototaxis:
Progress report: We have attempted transformations with promoter, RBS and terminator for our assembly again. Apparently there might be a problem with our LA plates. This time we have used competent e. coli MG1655. We will also use this strain for testing beta-carotene and retinal bricks.
A FedEx man is on his way as with our cDNA for the retinal generator as I write this. ETA some time tomorrow!
Working hypothesis: We are discussing whether we should keep the system in e. coli TOP 10 rather than moving to MG1655 as the biosynthesis machinery might be unnecessarily over expressing beta-carotene and retinal in amounts far beyond what our photo sensor needs to function.(although we will still do testing in both strains experimentally, in part to help further characterization of the Cambridge part)
--CKurtzhals 19:09, 13 July 2010 (UTC)
