"
Team:Panama/14 October 2010
From 2010.igem.org
(Difference between revisions)
Nikkibiotech (Talk | contribs) (→October 14) |
Nikkibiotech (Talk | contribs) (→October 14) |
||
| Line 7: | Line 7: | ||
We did a miniprep on the 11 colonies that grew in liquid LB medium with Amp. (The method we used was without columns because the kit ran out). | We did a miniprep on the 11 colonies that grew in liquid LB medium with Amp. (The method we used was without columns because the kit ran out). | ||
| - | Then, we ran a gel of 1% agarose to see if we could see a difference in the size of the linear band of the plasmid to be able to determine if it contains the insert ( | + | Then, we ran a gel of 1% agarose to see if we could see a difference in the size of the linear band of the plasmid to be able to determine if it contains the insert (Rh1AB) or not. |
[[Image:Cuadro14octubre.JPG|500px|thumb|left|alt text]] | [[Image:Cuadro14octubre.JPG|500px|thumb|left|alt text]] | ||
Latest revision as of 03:49, 28 October 2010
October 14
Prepared Ampicillin stock (50 mg/ml)
We did a miniprep on the 11 colonies that grew in liquid LB medium with Amp. (The method we used was without columns because the kit ran out). Then, we ran a gel of 1% agarose to see if we could see a difference in the size of the linear band of the plasmid to be able to determine if it contains the insert (Rh1AB) or not.
Miniprep Protocol:
For this procedure we used what is called a "dirty miniprep". And by dirty we don't mean that we did it with unclean utensils nor did we do it after coming from a soccer game; it means that the reagents were prepared in the lab and they weren't obtained from a commercial kit.
|
|
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
