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ULB/26 August 2010
From 2010.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
*Made the minipreps again. | *Made the minipreps again. | ||
*First ligations of the various pieces to build the two plasmids. | *First ligations of the various pieces to build the two plasmids. | ||
| + | |||
| + | '''Module hydrogen''' | ||
| + | * The test on the other deletion candidate are once again all negative | ||
| + | * We continue the purification process for the deletion candidate | ||
| + | * We continue to try to obtain other deletion candidates | ||
| + | |||
| + | '''Module homologous recombination''' | ||
| + | * We inserted the cm and kan resistance cassette into the pSB1C3 | ||
| + | |||
| + | '''Module heavy metals detection''' | ||
| + | * We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow | ||
| + | * We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow | ||
| + | |||
| + | '''Other''' | ||
| + | * We did a PCR on pSB1C3 in order to have some more | ||
Revision as of 03:10, 28 October 2010
Quorum addiction module
- The minipreps done on the 26 contained some genomic DNA, so we purified them on gel. Sadly, the purification didn't work, all the DNA was lost.
- Made the minipreps again.
- First ligations of the various pieces to build the two plasmids.
Module hydrogen
- The test on the other deletion candidate are once again all negative
- We continue the purification process for the deletion candidate
- We continue to try to obtain other deletion candidates
Module homologous recombination
- We inserted the cm and kan resistance cassette into the pSB1C3
Module heavy metals detection
- We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow
- We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow
Other
- We did a PCR on pSB1C3 in order to have some more
