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Team:UT-Tokyo/Sudoku notebook 6
From 2010.igem.org
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== '''Notebook''' == | == '''Notebook''' == | ||
Latest revision as of 03:00, 28 October 2010
Sudoku
Notebook => [ June / July / August / September - October ]
Notebook
6/23
Transformation
2010 Kit plate1: 6N,1H,5D,17J,4H,18A
2010 Kit plate2: 24C,2K
6/24
Inoculation
2010 Kit plate1: 6N,1H,5D,17J,4H,18A
2010 Kit plate2: 24C,2K
(6/23 Transformation)
6/25
Miniprep
2010 Kit plate1: 6N,1H,5D,17J,4H,18A
2010 Kit plate2: 24C,2K
(6/24 Inoculation)
6/27
Enxyme digestion
1~7, 9 & 10 plasmid
1: 117.2 ng/uL -> 2.56 uL / SP
2: 59.3 ng/uL -> 5.06 uL / SP
3: 124.2 ng/uL -> 2.42 uL / XP
4: 114.5 ng/uL -> 2.62 uL / EX
5: 81.3 ng/uL -> 3.69 uL / SP
6: 101.7 ng/uL -> 2.95 uL / ES
7: 81.5 ng/uL -> 3.68 uL / EX
9: 85.4 ng/uL -> 3.51 uL / SP
10: 73.5 ng/uL -> 4.08 uL / SP
6/28
Gel extract
1~7, 9 & 10 plasmid (6/27 enzyme digestion)
6 -> failed, others -> succeeded
6/29
Cutcheck
EScut: 1~5, 7, 9 & 10 (6/27 enzyme digestion)
HindIII cut: 6
6/30
Ligation
2-3
2: 5uL, 3: 5uL, ligation mix: 10uL
(6/27 Enzyme digestion)
Transformation
2010 Kit plate1: 1N (TetA(C) (Backwards))
2010 Kit plate2: 22P (OmpA)
2-3 (6/30 Ligation)
