Revision as of 02:58, 28 October 2010

Parts submitted

The Cambridge iGEM team has submitted 20 parts to the [http://partsregistry.org/Main_Page Registry of Standard Biological Parts]. Many of these parts have been extensively characterised. This data is stored in the Parts Registry, you can click on any BioBrick to view its technical datasheet.

Featured parts

BBa_K325259

Red luciferase and LRE operon from L. cruciata under pBAD

This part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, Luciola cruciata, when induced by L-arabinose. The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type. The sequence of nucleotides is codon optimised for expression in E. coli.

BBa_K325259

V. fischeri luxCDABE under pBAD

This part generates luxCDABE proteins from the bioluminescent bacterium, Vibrio fischeri, when induced by L-arabinose. The BioBrick produces light from basic metabolites found in E. coli, luxCDE produce the substrate used by luxAB for bioluminescence. The part creates a blue light.

Parts based on bacterial systems

BBa_K325902	V. fischeri luxCD This is a translational unit designed to produce V. fischeri luxC and luxD genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. Both proteins are part of the fatty aldehyde synthesis pathway. luxC is a reductase and luxD is a acyltransferase. LuxE must also be supplied to complete the pathway for fatty aldehyde synthesis. Such fatty aldehydes are the substrate for the luxAB bacterial luciferase.
BBa_K325903	V. fischeri luxEG This is a translational unit designed to produce V. fischeri luxE and luxG genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. luxE functions as the synthetase creating fatty aldehydes for bioluminescence. LuxG is a flavin reductase.
BBa_K325905	luxCDEG pLambda AB This part constitutively expresses [http://partsregistry.org/wiki/index.php?title=Part:BBa_K216008 the Xenorhabdus luminescens luxAB luciferase] already in the registry. It also features a translational unit for the substrate generating luxCDEG from Vibrio fischeri. This means that only when supplied with PoPs will it produce light output.
BBa_K325906	pBAD luxCDEG pLambda AB This is the same as the part above but was built for testing induction by adding the pBAD promoter, making light inducible by addition of L-arabinose.

Firefly luciferases

BBa_K325101	Luciferase from Photinus pyralis with increased substrate affinity This is a translational unit for a mutated and codon-optimised form of the luciferase from the North American firefly, Photinus pyralis. It is described in [http://www.ncbi.nlm.nih.gov/pubmed/17540326 Fujii et al. 2007] as having a 10 times higher substrate affinity and luminescence output compared to wildtype.
BBa_K325108	P.pyralis luciferase under pBAD This is the same as the part above but placed under the pBAD promoter induced by L-arabinose for characterisation.
BBa_K325211	L. cruciata luciferase (red mutant) This is a translational unit for a mutant (Ser286Asn) and codon-optimised luciferase from the Japanese firefly, Luciola cruciata. It is deeply red-shifted as compared to wild type.
BBa_K325218	L. cruciata luciferase under pBAD This is the same as the part above but placed under the pBAD promoter induced by L-arabinose for characterisation.

Firefly luciferin regenerating enzymes

BBa_K325102	P. pyralis Luciferin Regenerating Enzyme This is a translational unit for a codon-optimised Luciferin Regenerating Enzyme (LRE) from the North American firefly, Photinus pyralis.
BBa_K325202	L. cruciata Luciferin Regenerating Enzyme This is a translational unit for a codon-optimised Luciferin Regenerating Enzyme (LRE) from the Japanese firefly, Luciola cruciata.

Firefly luciferase, LRE operons

BBa_K325210	Luciola cruciata LRE and luciferase This translational unit combines the luciferin regenerating enzyme (BBa_K325202) and red-shifted luciferase (BBa_K325211) from the Japanese firefly, Luciola cruciata. The luciferin regenerating enzyme helps to strengthen and sustain light output.
BBa_K325100	Photinus pyralis LRE and luciferase This part combines the luciferin regenerating enzyme (BBa_K325102) and luciferase (BBa_K325101) from the North American firefly, Photinus pyralis. The luciferin regenerating enzyme helps to strengthen and sustain light output.
BBa_K325109	Photinus pyralis LRE and luciferase under pBAD This part is the part above expressed under pBAD for characterisation.
BBa_K325209	L. cruciata LRE and luciferase (wildtype) under pBAD This part combines the Luciferin Regenerating Enzyme and luciferase from the Japanese firefly, Luciola cruciata. It is expressed under pBAD.
BBa_K325229	L. cruciata LRE and luciferase (green) under pBAD This is the same as the part above but with a Val239Ile mutation which green-shifts its emission spectrum.
BBa_K325239	L. cruciata LRE and luciferase (red) under pBAD This is the same as the part above but instead has a Gly326Ser mutation which red-shifts its emission spectrum.
BBa_K325249	L. cruciata LRE and luciferase (scarlet) under pBAD This is the same as the part above but instead has a His433Tyr mutation which red-shifts its emission spectrum.
BBa_K325259	L. cruciata LRE and luciferase (yellow) under pBAD This is the same as the part above but instead has a Pro452Ser mutation which shifts its emission spectrum to peak at a yellow wavelength.

Summary table

<groupparts>iGEM010 Cambridge</groupparts>

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 {{:Team:Cambridge/Templates/TableRowAll|3d=259|image=a/a6/G28.jpg|title=Red luciferase and LRE operon from L. cruciata under pBAD|description=This part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, <i>Luciola cruciata</i>, when induced by L-arabinose.  The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type.  The sequence of nucleotides is codon optimised for expression in E. coli.}}
-{{:Team:Cambridge/Templates/Tablerow|3d=909|title=V. fischeri luxCDABE under pBAD|description=This part generates luxCDABE proteins from the bioluminescent bacterium, <i>Vibrio fischeri</i>, when induced by L-arabinose.  The BioBrick produces light from basic metabolites found in E. coli, luxCDE produce the substrate used by luxAB for bioluminescence.  The part creates a blue light.}}
+{{:Team:Cambridge/Templates/TableRowAll|3d=259|image=a/a6/G28.jpg|title=V. fischeri luxCDABE under pBAD|description=This part generates luxCDABE proteins from the bioluminescent bacterium, <i>Vibrio fischeri</i>, when induced by L-arabinose.  The BioBrick produces light from basic metabolites found in E. coli, luxCDE produce the substrate used by luxAB for bioluminescence.  The part creates a blue light.}}
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Team:Cambridge/BioBricks

From 2010.igem.org