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Team:Groningen/25 October 2010
From 2010.igem.org
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'''David, Arend Jan and Peter''' | '''David, Arend Jan and Peter''' | ||
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After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out | After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out | ||
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[[Image:ceramic coating gr1.jpg]] | [[Image:ceramic coating gr1.jpg]] | ||
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| + | '''Expression experiment David & Peter''' | ||
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| + | Massspec data for the induced cultures of E, EN, C and CN was aquired, with the help of Sander we performed Malditov mass spectometry on both these samples and the samples in stock from the large E induction experiment. The samples of the E-induction experiment gave no data. | ||
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| + | [[Image:MassSpecGR.jpg]] | ||
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Expression experiment: | Expression experiment: | ||
Latest revision as of 02:29, 28 October 2010
Week 43
David, Arend Jan and Peter
Growing transformants with GFP labeled SrfA and yqxM promotors. To see when the cell density dependant quorum sensing system kicks in to activate the promotors, sequential cultures were inoculated every our at an OD of 0.1. After 7 hours, the OD from the sequential cultures was measured together with the GFP fluorescence.
GFP fluorence:
OD mesearements:
David
Biofilm paste experiment
Growing biofilms on sterilized ceramics that have been coated with athin layer of TY medium with corn starch.
Mix: 30 mg cornstarch/ml TY 1% agar medium and 0.5% glucose
After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out
Expression experiment David & Peter
Massspec data for the induced cultures of E, EN, C and CN was aquired, with the help of Sander we performed Malditov mass spectometry on both these samples and the samples in stock from the large E induction experiment. The samples of the E-induction experiment gave no data.
Expression experiment:
ThT measurement of EH induced together with C non induced(this due to a mixup during he experiment), EH non induced, C induced
