Team:Queens-Canada/2 September 2010

From 2010.igem.org

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Hao
Hao
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No F1 worms from 02/09/10 injections.
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No transgenic F1 from 02/09/10 injections.
'''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3
'''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3

Latest revision as of 02:27, 28 October 2010

September 2, 2010

Miniprep

Steve and Chris

eYFP, eGFP, mCherry::unc-54 3'UTR::1A3

Digestion

Steve and Chris

eYFP, eGFP, mCherry::unc-54 3'UTR::1A3

Gel Electrophoresis

Chris and Steve

eYFP, eGFP, mCherry::unc-54 3'UTR::1A3

Microinjection

Hao

No transgenic F1 from 02/09/10 injections.

pStr-1::eCFP::unc-54 3'UTR::1A3, pOdr-1::eCFP::unc-54 3'UTR::1A3

Co-injected each construct with Sur-5::GFP marker.