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Contents 1 Notebook 1.1 6/16/10 1.2 6/19/10 1.3 6/21/10 1.4 6/23/10 1.5 6/24/10 1.6 6/25/10 1.7 6/25/10 1.8 6/28/10 1.9 6/29/10 1.10 7/1/10 1.11 7/6/10 1.12 7/12/10 1.13 7/13/10

Notebook

6/16/10

Prepared nine pots of wildtype (Col-0) A. thaliana and moved to cold storage for stratification. Pots intended for use in Agrobacterium flower-dip. Seeds were sewn in soilless media and the pot was covered with a wide meshing to retain the soil.

Prepared proliferative media for GBAM1 glioblastoma cell line. Began culturing cells (n = 14).

6/19/10

Divided GBAM1 cells into media for proliferation and differentiation.

6/21/10

Transfered pots from cold storage to greenhouse. Prepared 0.5 MS agar media and sterilized seeds for sterile growth, to be sewn on 6/23. Seeds left in dilute agar solution in fridge to stratify.

Replenished GBAM1 media. Selected cells for lactate flux sensing.

6/23/10

Agar plates poured. Seeds to be sewn on 6/24 to give time for the media to solidify.

6/24/10

Seeds sown onto agar. Five large culture plates and two small plates were then transfered into horticulture growth chamber. Seeds in soil media forecasted to germinate by 6/26.

Replenished GBAM1 media. Selected cells for oxygen flux sensing.

6/25/10

A. thaliana expirement mapping: Testing low oxygen response in WT and Transgenic using atmospheric chamber in Purdue Agronomy department. Using sensors to measure ethanol, oxygen, auxin response in control and in hypoxic conditions.

6/25/10

Communicating with IU iGEM team concerning synergies including arabidopsis transformation workshop.

Communicating with Naperville, Ill high school student as part of community outreach experience.

Planning Purdue University campus poster session to involve public in forum discussion on genetic engineering.

6/28/10

Sterilized seeds for plates. Potted seeds on soil, placed in cold room.

Transformed E. coli heat shock method.

6/29/10

Transformation of E. coli failed.

7/1/10

Poured agar. Placed new plants onto sterile plates. Placed in growth room. Placed potted plants in mist room.

7/6/10

Successful growth of all plants noted. One small mold colony present in plate.

7/12/10

Transformation of E. coli: attempt # 2

7/13/10

Transformation of E. coli: Success