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Team:Queens-Canada/26 July 2010
From 2010.igem.org
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'''pSB1A3''', '''pSB1T3''', '''pSB1K3''', '''pSB1C3''' | '''pSB1A3''', '''pSB1T3''', '''pSB1K3''', '''pSB1C3''' | ||
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<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
Latest revision as of 02:00, 28 October 2010
July 26, 2010
Gel Electrophoresis
Yuli
pSB1A3, pSB1A2
Used 0.35 g agarose in 50 mL TBE. Gel run for 45 mins at 100 V. Well were deformed as comb was pulled out prematurely.
PCR
Thai
pSB1A3, pSB1C3, pSB1T3, pSB1A2
Program run using 9:1 FAST:HIFI blend with an extension time of 70 s and annealing temperature of 65 °C.
Heat Shock
Yuli
pSB1A3, pSB1T3, pSB1K3, pSB1C3
Microinjection
Hao
Practiced injections with Sur-5::GFP marker.
