Team:Queens-Canada/2 September 2010
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(Difference between revisions)
(New page: <h2>September 2, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Steve and Chris '''eYFP''', '''eGFP''', '''mCherry'''::'''unc-54 3'UTR...) |
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<h2>September 2, 2010</h2> | <h2>September 2, 2010</h2> | ||
{{:Team:Queens-Canada/head}} | {{:Team:Queens-Canada/head}} | ||
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'''eYFP''', '''eGFP''', '''mCherry'''::'''unc-54 3'UTR'''::1A3 | '''eYFP''', '''eGFP''', '''mCherry'''::'''unc-54 3'UTR'''::1A3 | ||
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+ | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
+ | Hao | ||
+ | |||
+ | '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3 | ||
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+ | Co-injected each construct with pRF4 marker | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} |
Revision as of 01:27, 28 October 2010
September 2, 2010
Miniprep
Steve and Chris
eYFP, eGFP, mCherry::unc-54 3'UTR::1A3
Digestion
Steve and Chris
eYFP, eGFP, mCherry::unc-54 3'UTR::1A3
Gel Electrophoresis
Chris and Steve
eYFP, eGFP, mCherry::unc-54 3'UTR::1A3
Microinjection
Hao
pStr-1::eCFP::unc-54 3'UTR::1A3, pOdr-1::eCFP::unc-54 3'UTR::1A3
Co-injected each construct with pRF4 marker