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Team:Queens-Canada/8 July 2010
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(Difference between revisions)
(New page: <h2>July 8, 2010</h2> {{:Team:Queens-Canada/head}} <h3>PCR</h3> Thai '''pGpd-2''', '''pFlp-1''' Program run using HIFI with an extension time of 25 s...) |
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Cut using EcoRI and PstI. | Cut using EcoRI and PstI. | ||
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| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao and Yuli | ||
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| + | Practiced injections with water. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 01:13, 28 October 2010
July 8, 2010
PCR
Thai
pGpd-2, pFlp-1
Program run using HIFI with an extension time of 25 s and annealing temperature of 57 °C.
Heat Shock
Chris
pBluescriptII
Plates stored in 37 °C room overnight.
Gel Electrophoresis
Thai and Yuli
Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.
Gel Electrophoresis
Hao and Chris
Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.
Digestion
Yuli
BBa_I13522
Cut using EcoRI and PstI.
Microinjection
Hao and Yuli
Practiced injections with water.
