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Team:Stanford/Research/Modeling
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==Kinase/Phosphatase System== | ==Kinase/Phosphatase System== | ||
| - | [[Image: | + | [[Image:MainGBK.jpg]] |
| - | From Michaelis-Menten kinetics we know that the rate at which | + | From Michaelis-Menten kinetics we know that the rate at which Zp is dephosphorylated is [[Image:R1.jpg]] |
and the rate at which Z is phosphorylated is | and the rate at which Z is phosphorylated is | ||
| - | [[Image: | + | [[Image:R2.jpg]] |
</div> | </div> | ||
Revision as of 01:07, 28 October 2010
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Contents |
Goals
Our intuition for what makes a good ratio sensor could only take us so far. From the very first stages of design, we wanted to back up and test our ideas with mathematical tools. Luckily, we found that solving the equations of mass action kinetics at steady-state was enough to give us clear design criteria. We present the mathematical basis for sensors that are capable of sensing a single ratio digitally, or many ratios in an analog fashion.
The System
sRNA System
Boolean model for the sRNA system. This is mean to provide a significantly simplified mathematical understanding of the dynamics involved.
clear all clc %Constants and Values trackingNum = 6; %mRNA_pA, mRNA_pB, sRNA_A, sRNA_B, protein_A, protein_B; inputNum = 2; %A, B; processNum = 6; %Trx_pA, Trx_sRNA_A, Trx_pB, Trx_sRNA_B, Trl_A, Trl_B; %Cell/Matrix Dimensions m = 2^trackingNum; n = 2^inputNum; c = cell(m+1, n+1); trackingMatrix = de2bi(0:m-1); inputMatrix = de2bi(0:n-1); processMatrix = zeros(1,processNum); %Populating the Cell (Tracking x Input) c{1,1} = [0]; for j = 1 for i = 2:m+1 c{i,j} = trackingMatrix(i-1,:); end end for i = 1 for j = 2:n+1 c{i,j} = inputMatrix(j-1,:); end end for i = 2:m+1 for j = 2:n+1 c{i,j} = processMatrix; end end %Rules, Acessing, and Changing for j = 2:n+1 A = c{1,j}(1); B = c{1,j}(2); for i = 2:m+1 mRNA_pA = c{i,1}(1); mRNA_pB = c{i,1}(2); sRNA_A = c{i,1}(3); sRNA_B = c{i,1}(4); protein_A = c{i,1}(5); protein_B = c{i,1}(6); %Trx_pA if A == 1 c{i,j}(1) = 1; end %Trx_pB if B == 1 c{i,j}(2) = 1; end %Trx_sRNA_A if A == 1 c{i,j}(3) = 1; end %Trx_sRNA_B if B == 1 c{i,j}(4) = 1; end %Trl_A if mRNA_pA == 1 && mRNA_pB == 0 c{i,j}(5) = 1; end %Trl_B if mRNA_pB == 1 && mRNA_pA == 0 c{i,j}(6) = 1; end end end %Display Results (Column by Column) [nrows,ncols]= size(c); %Condense Cell and Display for i = 1:nrows for j = 1:ncols string = num2str(c{i,j}); l = length(string); r = 1; s = 1; t = 0; while t ~= 1 if r == l t = 1; end noSpacesString(s) = string(r); r = r+3; s = s+1; end c{i,j} = noSpacesString; end end c(:,:) %Find Steady States and Corresponding Inputs counter = 1; for i = 2:m+1 for j = 2:n+1 if c{i,j} == c{i,1} completeMatrix{counter,1} = c{i,j}; %Steady state values completeMatrix{counter,2} = num2str([i-1,j-1]); %Location (n x m) within results area completeMatrix{counter,3} = c{1,j}; %Corresponding input values counter = counter+1; end end end SS_mXn_Input = completeMatrix time = 0:processNum; %plot(time,,time,,time,,time,,time,,time,) %New Cell for Specific Cases % d(:,1) = c(:,1); % for j = 2:n+1 % C1 = '0000'; % C2 = '0011'; % C3 = '0100'; % C4 = '0111'; % C5 = '1000'; % C6 = '1011'; % C7 = '1100'; % % a = {C1, C2, C3, C4, C5, C6, C7}; % for b = 1:1:length(a) % if strcmp(c(1,j),a(b)) == 1 % d(:,b+1) = c(:,j); % end % end % end % d
ans =
'0' '00' '01' '10' '11'
'000000' '000000' '010100' '101000' '111100'
'000001' '000000' '010100' '101000' '111100'
'000010' '000000' '010100' '101000' '111100'
'000011' '000000' '010100' '101000' '111100'
'000100' '000000' '010100' '101000' '111100'
'000101' '000000' '010100' '101000' '111100'
'000110' '000000' '010100' '101000' '111100'
'000111' '000000' '010100' '101000' '111100'
'001000' '000000' '010100' '101000' '111100'
'001001' '000000' '010100' '101000' '111100'
'001010' '000000' '010100' '101000' '111100'
'001011' '000000' '010100' '101000' '111100'
'001100' '000000' '010100' '101000' '111100'
'001101' '000000' '010100' '101000' '111100'
'001110' '000000' '010100' '101000' '111100'
'001111' '000000' '010100' '101000' '111100'
'010000' '000001' '010101' '101001' '111101'
'010001' '000001' '010101' '101001' '111101'
'010010' '000001' '010101' '101001' '111101'
'010011' '000001' '010101' '101001' '111101'
'010100' '000001' '010101' '101001' '111101'
'010101' '000001' '010101' '101001' '111101'
'010110' '000001' '010101' '101001' '111101'
'010111' '000001' '010101' '101001' '111101'
'011000' '000001' '010101' '101001' '111101'
'011001' '000001' '010101' '101001' '111101'
'011010' '000001' '010101' '101001' '111101'
'011011' '000001' '010101' '101001' '111101'
'011100' '000001' '010101' '101001' '111101'
'011101' '000001' '010101' '101001' '111101'
'011110' '000001' '010101' '101001' '111101'
'011111' '000001' '010101' '101001' '111101'
'100000' '000010' '010110' '101010' '111110'
'100001' '000010' '010110' '101010' '111110'
'100010' '000010' '010110' '101010' '111110'
'100011' '000010' '010110' '101010' '111110'
'100100' '000010' '010110' '101010' '111110'
'100101' '000010' '010110' '101010' '111110'
'100110' '000010' '010110' '101010' '111110'
'100111' '000010' '010110' '101010' '111110'
'101000' '000010' '010110' '101010' '111110'
'101001' '000010' '010110' '101010' '111110'
'101010' '000010' '010110' '101010' '111110'
'101011' '000010' '010110' '101010' '111110'
'101100' '000010' '010110' '101010' '111110'
'101101' '000010' '010110' '101010' '111110'
'101110' '000010' '010110' '101010' '111110'
'101111' '000010' '010110' '101010' '111110'
'110000' '000000' '010100' '101000' '111100'
'110001' '000000' '010100' '101000' '111100'
'110010' '000000' '010100' '101000' '111100'
'110011' '000000' '010100' '101000' '111100'
'110100' '000000' '010100' '101000' '111100'
'110101' '000000' '010100' '101000' '111100'
'110110' '000000' '010100' '101000' '111100'
'110111' '000000' '010100' '101000' '111100'
'111000' '000000' '010100' '101000' '111100'
'111001' '000000' '010100' '101000' '111100'
'111010' '000000' '010100' '101000' '111100'
'111011' '000000' '010100' '101000' '111100'
'111100' '000000' '010100' '101000' '111100'
'111101' '000000' '010100' '101000' '111100'
'111110' '000000' '010100' '101000' '111100'
'111111' '000000' '010100' '101000' '111100'
SS_mXn_Input =
'000000' '1 1' '00'
'010101' '22 2' '01'
'101010' '43 3' '10'
'111100' '61 4' '11'
Kinase/Phosphatase System
From Michaelis-Menten kinetics we know that the rate at which Zp is dephosphorylated is File:R1.jpg and the rate at which Z is phosphorylated is File:R2.jpg
