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Team:Queens-Canada/28 July 2010
From 2010.igem.org
(New page: <h2>July 28, 2010</h2> {{:Team:Queens-Canada/head}} <h3>PCR</h3> Hao '''pSip-1''', '''pRab-7''' Program run using 9:1 FAST:HIFI blend with an extens...) |
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'''pSrb-6''', '''pCeh-12''', '''pOdr-10''', '''NpHR''', '''ChR2''', '''pOsm-10''', '''pStr-1''', '''eCFP''', '''pHsp-3''', '''pFlp-1''', '''pSB1A3''', '''pStr-220''' | '''pSrb-6''', '''pCeh-12''', '''pOdr-10''', '''NpHR''', '''ChR2''', '''pOsm-10''', '''pStr-1''', '''eCFP''', '''pHsp-3''', '''pFlp-1''', '''pSB1A3''', '''pStr-220''' | ||
Latest revision as of 00:35, 28 October 2010
July 28, 2010
PCR
Hao
pSip-1, pRab-7
Program run using 9:1 FAST:HIFI blend with an extension time of 6 s and annealing temperature of 58 °C.
Gel Electrophoresis
Yuli and Steve
Used 1 g agarose in 140 mL TBE. Gel run for 35 mins at 100 V.
Digestion
Hao
pSB1A3
Half the samples were cut with EcoRI & SpeI, and the other half were cut with XbaI and PstI.
Gel Electrophoresis
Hao and Steve
pSB1A3
Used 0.5 g agarose in 70 mL TBE. Gel run for 40 mins at 100 V.
Digestion-Ligation
Steve and Thai
pRab-7, pSip-1, pMec-7, pSB1A3
Cut with XbaI and PstI. Backbone treated with CIAP.
Digestion
Hao and Nelson
pSrb-6, pCeh-12, pOdr-10, NpHR, ChR2, pOsm-10, pStr-1, eCFP, pHsp-3, pFlp-1, pSB1A3, pStr-220
Cut with EcoRI and PstI. May have damaged spin column filter of pHsp-3 during PCR purification. Transferred flow-through to new spin column. Old spin column may have trapped the DNA. Finished PCR purification with both columns.
