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Team:TU Delft/12 July 2010 content
From 2010.igem.org
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Revision as of 22:33, 12 July 2010
Lab Work
Characterization of Anderson RBS sequences
E.coli TOP 10 strains containing the following composite biobricks in pSB1A2 had been obtained:
| BioBrick # | Composed of |
| [http://partsregistry.org/Part:BBa_K398050 K398050] | J23100 + J61100 + I13401 |
| K398051 [http://partsregistry.org/Part:BBa_K398051] | J23100 + J61101 + I13401 |
| K398052 [http://partsregistry.org/Part:BBa_K398052] | J23100 + J61107 + I13401 |
| K398053 [http://partsregistry.org/Part:BBa_K398053] | J23100 + J61117 + I13401 |
| K398054 [http://partsregistry.org/Part:BBa_K398054] | J23100 + J61127 + I13401 |
To prepare for the fluorescence assay measurements the strains were grown in M9 minimal medium containing 0.4% glucose and ampicillin.
High copy number RBS characterizations are rarely indicative of RiPS in system operation plasmids, thus the fluorescence assays will also be performed with the biobricks on pSB3C5. In order to obtain sufficient BioBrick DNA the strains were grown in 250 mL LB-Amp, awaiting isolation and plasmid swapping.
