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Revision as of 23:33, 27 October 2010

Your promoter and Your protein

Project overview by figure.

In the overview figure of our project, you can see your promoter and your protein. Because the main purpose of our project is to provide a speedy and useful tool for everyone who is doing synthetic biology research, we designed a flexible system which can be applied for different needs. The explanation of your promoter and your protein is as below:

• Your promoter

Here you can change "your promoter" with your own promoter which you are interested in. This part can be used to measure the quantity and locations of mRNA induced by different types or different strength promoters. With this novel method, we can get faster and more understanding the relationship between promoters and mRNA transcription.

• Your protein

Here you can also change to the use of your own protein and by fusion with our specific peptide adaptor. Then you can use the novel speedy protein reporter in our SpeedyBac system to know the localization of different proteins and the influence of translation under different situations.

Applications

Our project can achieve several benefits and have several applications as follows:

• Our SpeedyBac can be used to measure the gene expression in single cells. It helps us to avoid the influence from population.
• Users can change to he use of their own promoters and proteins easily and measure them much faster than traditional methods.
• With our Speedy switch, we can stop the gene expression at transcription, so we can study the roles of mRNA in gene expression more easily.
• Comparison with the traditional methods of measuring gene expression, our SpeedyBac provide a faster and more flexible way in studying the gene expression and interactions among different biological parts in vivo.