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Team:TU Delft/8 July 2010 content
From 2010.igem.org
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Yesterday's ligation products containing I13401 and the 5 Anderson RBS plasmids (J61100, J61101, J51107, J61117, J61127) yielded successful transformants! The single colony PCRs performed indicated the presence of inserts with the proper lengths. These colonies were further plated out to yield reincultures for subsequent characterization. | Yesterday's ligation products containing I13401 and the 5 Anderson RBS plasmids (J61100, J61101, J51107, J61117, J61127) yielded successful transformants! The single colony PCRs performed indicated the presence of inserts with the proper lengths. These colonies were further plated out to yield reincultures for subsequent characterization. | ||
Revision as of 20:09, 12 July 2010
Lab work
Today we got the Gas Chromatograph working, we could identify several peaks. Now we are ready for the real experiments!
We harvested the 200 mL bacterial cells of the 16 DNA parts. We used 3 mL of the bacterial cells to make -80 °C stocks. With the rest we centrifuged at 4,000 rmp for 15 minutes and stored the pellets in the -20 °C.
Characterization of Anderson RBS sequences
Yesterday's ligation products containing I13401 and the 5 Anderson RBS plasmids (J61100, J61101, J51107, J61117, J61127) yielded successful transformants! The single colony PCRs performed indicated the presence of inserts with the proper lengths. These colonies were further plated out to yield reincultures for subsequent characterization.
