Team:HokkaidoU Japan/Notebook/October4

From 2010.igem.org

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{{Template:HokkaidoU_Japan}}
<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/October3|October 3]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/October5|October 5]]</div></div>
<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/October3|October 3]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/October5|October 5]]</div></div>
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時間が無くて，これ以降の記録無し．
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Sequencing of 2 samples, RBS + Arabinose
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{|border="5"
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| 9:00
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*Reserve the sequencer for use at 16:00 if posible
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| 12:10
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*Consult colonie PCR to choose constructs for Sequencing.
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*Miniprep
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*MasterMix
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*PCR for sequencing, use 100 up and 200 down primers
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| 12:55~14:55
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*PCR
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| 15:00~15:40
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*Ethanol precipitation
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*Melting in Hi-DI
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| 16:00
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*Start sequencing
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| 17:00
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*Retrieve sequence
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Latest revision as of 22:28, 27 October 2010

Notebook
時間が無くて，これ以降の記録無し．
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Notebook/October4"