Team:Cambridge/Templates/boxesprototypenew

From 2010.igem.org

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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">We investigated the capacity of bioluminescence for the generation of sustainable alternatives to traditional light sources. We placed genes form <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">prokaryotes</a> into E.coli to make E.glowli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours.</p>
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> We placed genes from <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">prokaryotes</a> into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/Lighting">biological alternatives</a> to conventional lighting and quantitative reporters for <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/microMeasure">biosensors</a>.</p>
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Revision as of 22:03, 27 October 2010

Abstract

We placed genes from fireflies and bioluminescent prokaryotes into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include biological alternatives to conventional lighting and quantitative reporters for biosensors.

Multimedia

If you want a break from hard-core science, check out our Gibson Assembly music video.

You can also see view videos of our bacterial bubble lamp and project overview.