Team:Newcastle/Meetings/12 July 2010

From 2010.igem.org

(Difference between revisions)
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#Cloning Strategies  
#Cloning Strategies  
#*YnEA
#*YnEA
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#*One sequence for sfp ready  
+
#*One sequence for sfp primers and coding sequence ready (Goksel)
-
# Finish Wiki updates Sunday 18th
+
#*Subtilin just immunity
 +
#Finish Wiki updates Sunday 18th
 +
#Add to list of advisors: Colin Harwood, Colin Daviy, Wendy Smith and Goksel Misrili

Revision as of 15:42, 12 July 2010

Chair: Da, Minutes: Younus, Comp

  1. . Cloning Strategies
  • SR1 - Finished
  • RocF not started - need to use Gibson method;
  • YneA
  • 2 for Swarming (sfp and swrA)
  • LacI
  • 2 for Subtilin (Immunity and Production)

Only finished one, SR1. 7 more to go!

Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test. Why is testing LacI hard?

  • YneA - our most important BB - Phil, Rach, Deena, Younus
  • Swarming (2) - Harsh, Alan, Steve, Da
  • LacI - Phil, Younus, Rachel
  • Subtilin (2) - Harsh, Alan, Steve, Deena
  • RocF - Harsh, Alan, Steve, Da

5 parts to a Cloning Strategy

  1. Purpose and justification
  2. Modelling
  3. Construction
  4. Cloning and Integration
  5. Testing and Characterising


  1. . Wiki

Labbook

  1. . Aim
  2. . Materials and Methods
  3. . Procedure/Protocol
  4. . Results
  5. . Conclusion

Everyone needs to carry out this protocol above Photographs add and label them In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)



20th July - UK iGEM Gathering at Newcastle!

PRESENTATION Introduction - Rach, Deena, Jannetta

  • Cover Page
  • Picture of standing us up
  • Introduction of our iGEM project - using BS to repair concrete (2 mins)


General Outlook (Harsh, Da, Phil) (5 mins):

  • Starting with bioreactor
  • Transformation into spores
  • Transportation
  • Spraying on building
  • Germination
  • sensing crack + non-environmental kill switch
  • swarming
  • end of crack signalling = QS
  • Filamentous cells
  • CaCO3
  • Glue
  • Kill Switch

BioBrick Discussion (Alan, Younus, Steve) (8 mins altogether; 2 mins each):

  • Swarming
  • Subtillin
  • Filamentous cells - YneA
  • CaCo3 - Urease

(5 mins remaining)

Whats next... 'Save the babies!!!'


Minideadlines:

  1. Cloning Strategies
    • YnEA
    • One sequence for sfp primers and coding sequence ready (Goksel)
    • Subtilin just immunity
  2. Finish Wiki updates Sunday 18th
  3. Add to list of advisors: Colin Harwood, Colin Daviy, Wendy Smith and Goksel Misrili