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Team:Michigan/Quorum Sensing
From 2010.igem.org
(Difference between revisions)
| Line 48: | Line 48: | ||
Made CaCl<sub>2</sub> stock solution: 0.1M - 50mL | Made CaCl<sub>2</sub> stock solution: 0.1M - 50mL | ||
*molar mass = 111 g/mol | *molar mass = 111 g/mol | ||
| - | * | + | *0.1 mol/L)(.05 L)(111 g/mol) = 555mg |
*dissolved 555 mg CaCl<sub>2</sub> in 50mL DIwater | *dissolved 555 mg CaCl<sub>2</sub> in 50mL DIwater | ||
*vacuum filtered into 50mL tube | *vacuum filtered into 50mL tube | ||
Made ampicillin stock solution: | Made ampicillin stock solution: | ||
| - | + | *1 g ampicillin | |
| - | + | *5mL DIwater | |
| - | + | *ethanol | |
*filtered by syringe into 15mL tube | *filtered by syringe into 15mL tube | ||
*put into ten 1mL alliquots in 1.5mL tubes | *put into ten 1mL alliquots in 1.5mL tubes | ||
| Line 67: | Line 67: | ||
Made LB agar plates w/ ampicillin | Made LB agar plates w/ ampicillin | ||
| - | + | *10 g LB broth | |
| - | + | *7.5 g agar | |
| - | + | *500 mL DI water | |
*autoclaved mixture at 121°C for 30 min (sterilize time), following Mike Nelson's protocol | *autoclaved mixture at 121°C for 30 min (sterilize time), following Mike Nelson's protocol | ||
---- | ---- | ||
Revision as of 14:00, 12 July 2010
| Sunday | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | |
| Week 1 | - | - | - | - | - | - | - |
| Week 2 | - | - | - | 7/7/2010 | 7/8/2010 | - | - |
| Week 3 | - | - | - | - | - | - | - |
Quorum Sensing Team
Members include Alex Pyden, Marcus Lehr, Jennifer Hong, Eric Raynal, Katie Miskovich, and Audra Williams
7/7/10
Jennifer and Alex - in Lin Lab
Made CaCl2 stock solution: 0.1M - 50mL
- molar mass = 111 g/mol
- 0.1 mol/L)(.05 L)(111 g/mol) = 555mg
- dissolved 555 mg CaCl2 in 50mL DIwater
- vacuum filtered into 50mL tube
Made ampicillin stock solution:
- 1 g ampicillin
- 5mL DIwater
- ethanol
- filtered by syringe into 15mL tube
- put into ten 1mL alliquots in 1.5mL tubes
- stored in -20°C in ERB lab
Inoculated E. coli DH5α from frozen stock into 12 mL LB broth
- put in 30°C, 200 rpm shaking, overnight
~1.5 hrs
7/8/10
Alex, Jennifer and Eric - in ERB
Made LB agar plates w/ ampicillin
- 10 g LB broth
- 7.5 g agar
- 500 mL DI water
- autoclaved mixture at 121°C for 30 min (sterilize time), following Mike Nelson's protocol
Created protocols for Obtaining Deionized Water in the ERB and ERB Spectrophotometer
- uploaded to Team:Michigan/Protocols section
poured 20 LB-amp plates (large)
- left to solidify in ERB 1230
- 12 plates were used by Ann for biobrick transformations
- 8 plates were stored in 4°C ERB
~3.5 hrs work
