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Team:Newcastle/21 June 2010
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'''Monday''' | '''Monday''' | ||
| - | + | ==Wet Lab techniques practice== | |
| - | + | ===Aims=== | |
The aim of today's Lab training session was to practice aseptic technique and wet lab techniques such as streak plating and broth culture. | The aim of today's Lab training session was to practice aseptic technique and wet lab techniques such as streak plating and broth culture. | ||
| - | + | ===Equipement list=== | |
| - | For today's session we needed | + | '''For today's session we needed''': |
* Agar plates | * Agar plates | ||
| Line 20: | Line 20: | ||
* Orbital shaker | * Orbital shaker | ||
| - | + | ===Tips=== | |
* Use Aseptic technique: Treat everything as a pathogen! | * Use Aseptic technique: Treat everything as a pathogen! | ||
* Work around the bunsen burner. | * Work around the bunsen burner. | ||
Revision as of 08:57, 12 July 2010
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Monday
Contents |
Wet Lab techniques practice
Aims
The aim of today's Lab training session was to practice aseptic technique and wet lab techniques such as streak plating and broth culture.
Equipement list
For today's session we needed:
- Agar plates
- Pipettes
- Wire loops
- E.coli (from a colony)
- LB broth
- Bunsen burner
- Conical flasks
- Orbital shaker
Tips
- Use Aseptic technique: Treat everything as a pathogen!
- Work around the bunsen burner.
- Use a control.
- Clean the bench at the end of the day!
- Heat the wire loop from the middle to the tip.
- Keep plates upside down.
- Keep lids off for as short a time as possible.
- Loosen all the tops off the vessels before you start.
- Flame tops of bottles lightly.
Streak plating
Star plate streaking student, Deena, shows off how it is to be done!
Set up culture for minipreps
5ml culture with E.coli from a single colony grown over night. Tomorrow we will harvest the DNA.
   
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