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Team:Michigan/Quorum Sensing
From 2010.igem.org
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| - | =Quorum Sensing Team | + | __NOTOC__ |
| + | {{Michigan Header}} | ||
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| + | {|style="color:#1c2bf2;background-color:#fafa19;font-size:9pt;text-align:center" cellpadding="5" cellspacing="0" border="1" bordercolor="#fff" width="62%" | ||
| + | |- | ||
| + | ! | ||
| + | |Sunday | ||
| + | |Monday | ||
| + | |Tuesday | ||
| + | |Wednesday | ||
| + | |Thursday | ||
| + | |Friday | ||
| + | |Saturday | ||
| + | |- | ||
| + | !Week 1 | ||
| + | | - | ||
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| + | !Week 2 | ||
| + | | - | ||
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| + | | [[Team:Michigan/Quorum_Sensing#7/7/2010|7/7/2010]] | ||
| + | | [[Team:Michigan/Quorum_Sensing#7/8/2010|7/8/2010]] | ||
| + | | - | ||
| + | | - | ||
| + | |- | ||
| + | !Week 3 | ||
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| + | |} | ||
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| + | =Quorum Sensing Team= | ||
Members include [[User:Infekt|Alex Pyden]], [[User:Chillywings|Marcus Lehr]], [[User:Jejihong|Jennifer Hong]], [[User:Ericrayn|Eric Raynal]], [[User:Katemisk|Katie Miskovich]], and [[User:Audraw|Audra Williams]] | Members include [[User:Infekt|Alex Pyden]], [[User:Chillywings|Marcus Lehr]], [[User:Jejihong|Jennifer Hong]], [[User:Ericrayn|Eric Raynal]], [[User:Katemisk|Katie Miskovich]], and [[User:Audraw|Audra Williams]] | ||
==7/7/10== | ==7/7/10== | ||
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~1.5 hrs | ~1.5 hrs | ||
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== 7/8/10 == | == 7/8/10 == | ||
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~3.5 hrs work | ~3.5 hrs work | ||
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Revision as of 19:08, 11 July 2010
| Sunday | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | |
| Week 1 | - | - | - | - | - | - | - |
| Week 2 | - | - | - | 7/7/2010 | 7/8/2010 | - | - |
| Week 3 | - | - | - | - | - | - | - |
Quorum Sensing Team
Members include Alex Pyden, Marcus Lehr, Jennifer Hong, Eric Raynal, Katie Miskovich, and Audra Williams
7/7/10
Jennifer and Alex - in Lin Lab
Made CaCl2 stock solution: 0.1M - 50mL
- molar mass = 111 g/mol
- (.1 mol/L)(.05 L)(111 g/mol) = 555mg
- dissolved 555 mg CaCl2 in 50mL DIwater
- vacuum filtered into 50mL tube
Made ampicillin stock solution:
-1 g ampicillin -5mL DIwater -ethanol
- filtered by syringe into 15mL tube
- put into ten 1mL alliquots in 1.5mL tubes
- stored in -20°C in ERB lab
Inoculated E. coli DH5α from frozen stock into 12 mL LB broth
- put in 30°C, 200 rpm shaking, overnight
~1.5 hrs
7/8/10
Alex, Jennifer and Eric - in ERB
Made LB agar plates w/ ampicillin
-10 g LB broth -7.5 g agar -500 mL DI water
- autoclaved mixture at 121°C for 30 min (sterilize time), following Mike Nelson's protocol
Created protocols for Obtaining Deionized Water in the ERB and ERB Spectrophotometer
- uploaded to Team:Michigan/Protocols section
poured 20 LB-amp plates (large)
- left to solidify in ERB 1230
- 12 plates were used by Ann for biobrick transformations
- 8 plates were stored in 4°C ERB
~3.5 hrs work
