Week of 8/9

From 2010.igem.org

(Difference between revisions)
(August 10th & 11th)
 
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<center>[[Image:8-11-10.jpg]]</center>
*Ligations:
*Ligations:

Latest revision as of 18:09, 27 October 2010

August 10th & 11th

  • Grew up Lux Promoters (R0061, R0063, K091146, K145150, K091107, R1062)
  • Made SOB media
  • Minipreps:
- K091107, K091146, R0063, R1062, K145150
- R0061 did not grow 
- LP(RFP+RBS) + 2 minipreps of RFP+RBS

  • Digests:
- Lux promoters with S+P
- RFP+RBS with E+P and X+P(Phosphatased)
- Digested K3+LP with E+P, K3 was phosphatased

  • Gel order:
-Ladder
-K091107
-R0063
-R1062
-K091146
-K145150
-RFP+RBS (EP)
-RFP+RBS (XP)
-K3
-LP
8-11-10.jpg
  • Ligations:
- RFP+RBS + Lux promoters
- K3 + LP
- K3 + RFP+RBS


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