Team:Harvard/parts

From 2010.igem.org

(Difference between revisions)
Line 101: Line 101:
<tr><td></td><td>BBa_K382041</td><td><i>Arabidopsis</i>-optimized Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus.  Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr>
<tr><td></td><td>BBa_K382041</td><td><i>Arabidopsis</i>-optimized Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus.  Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr>
-
<tr><td></td><td>BBa_K382050</td><td><i>Arabidopsis</i>-optimized Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
+
<tr><td>V21</td><td>BBa_K382050</td><td><i>Arabidopsis</i>-optimized Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
-
<tr><td></td><td>BBa_K382051</td><td><i>Arabidopsis</i>-optimized Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td>kan</td><td>nptII</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
+
<tr><td>V22</td><td>BBa_K382051</td><td><i>Arabidopsis</i>-optimized Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td>kan</td><td>nptII</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
-
<tr><td></td><td>BBa_K382052</td><td><i>Arabidopsis</i>-optimized Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
+
<tr><td>V25</td><td>BBa_K382052</td><td><i>Arabidopsis</i>-optimized Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
-
<tr><td></td><td>BBa_K382053</td><td><i>Arabidopsis</i>-optimized Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td>kan</td><td>nptII</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
+
<tr><td>V26</td><td>BBa_K382053</td><td><i>Arabidopsis</i>-optimized Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td>kan</td><td>nptII</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
-
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr>
+
<tr><td>V29</td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr>
-
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan</td><td>nptII</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr>
+
<tr><td>V30</td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan</td><td>nptII</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr>
-
<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan</td><td>pat</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">link</a></td></tr>
+
<tr><td>V33</td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan</td><td>pat</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">link</a></td></tr>
-
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan</td><td>pat</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">link</a></td></tr>
+
<tr><td>V35</td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan</td><td>pat</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">link</a></td></tr>
-
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan</td><td>pat</td><td><a href="https://static.igem.org/mediawiki/2010/0/0f/LYC.txt">link</a></td></tr>
+
<tr><td>V37</td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan</td><td>pat</td><td><a href="https://static.igem.org/mediawiki/2010/0/0f/LYC.txt">link</a></td></tr>
-
<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan</td><td>nptII</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">link</a></td></tr>
+
<tr><td>V34</td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan</td><td>nptII</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">link</a></td></tr>
-
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan</td><td>nptII</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">link</a></td></tr>
+
<tr><td>V36</td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan</td><td>nptII</td><td><a href="https://static.igem.org/mediawiki/2010/d/da/Beta-ophase.txt">link</a></td></tr>
-
<tr><td></td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan</td><td>nptII</td><td><a href="https://static.igem.org/mediawiki/2010/0/0f/LYC.txt">link</a></td></tr>
+
<tr><td>V38</td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan</td><td>nptII</td><td><a href="https://static.igem.org/mediawiki/2010/0/0f/LYC.txt">link</a></td></tr>
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan</td><td>pat</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">link</a></td></tr>
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan</td><td>pat</td><td> <a href="https://2010.igem.org/Team:Harvard/allergy/LTP.txt">link</a></td></tr>

Revision as of 18:01, 27 October 2010




parts

notebook notationbiobrick nameoriginal namedescriptionresistance (bacteria)resistance (plants)sequence
V7BBa_K382000pORE Open Series Vector with BioBrick SitesAgrobacterium vector open series with biobrick cloning sitekanpatlink
V8BBa_K382001pORE Open Series Vector with BioBrick SitesAgrobacterium vector open series with biobrick cloning sitekannptIIlink
V9BBa_K382002pORE Expression Series Vector with BioBrick SitesAgrobacterium vector with ENTCUP2 promoter and biobrick cloning sitekanpatlink
V10BBa_K382003pORE Expression Series Vector with BioBrick SitesAgrobacterium vector with ENTCUP2 promoter and biobrick cloning sitekannptIIlink
V11BBa_K382004pORE Reporter Series Vector with BioBrick SitesAgrobacterium vector with gusA reporter and biobrick cloning sitekannptIIlink
V12BBa_K382005pORE Reporter Series Vector with BioBrick SitesAgrobacterium vector with smgfp reporter and biobrick cloning sitekannptIIlink
C1BBa_K382010amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownchloramphenicol-link
C2BBa_K382011amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-link
C3BBa_K382012amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-link
A2BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructamp-link
A3BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-link
BBa_K382017amiRNA LTP LTP knockdown constructamp- link
BBa_K382018PDK intron hairpinIntron hairpin constructamp-link
BBa_K382019ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-link
F1BBa_K382020Arabidopsis-optimized BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-link
F2BBa_K382021Arabidopsis-optimized MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-link
F3BBa_K382022pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-link
F4BBa_K382023NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-link
F5BBa_K382024NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on 5' end.chloramphenicol-link
F6BBa_K382025Arabidopsis-optimized Miraculin StrepII N-terminusMiraculin with StrepII tag on N-terminus for Western Blot.chloramphenicol-link
F7BBa_K382026Arabidopsis-optimized Brazzein StrepII N-terminusBrazzein with StrepII tag on N-terminus for Western Blot.chloramphenicol-link
F8BBa_K382027Arabidopsis-optimized Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
F9BBa_K382028Arabidopsis-optimized Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
E2BBa_K382030RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.chloramphenicol-link
E3BBa_K382031RXRLc Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozideamp-link
E5BBa_K382033Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide chloramphenicol-link
E6BBa_K382034Act2pLacOActin promoter with LacO sites amp-link
BBa_K382035LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-link
BBa_K382036RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.amp-link
BBa_K382037Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide amp-link
BBa_K382040Arabidopsis-optimized Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
BBa_K382041Arabidopsis-optimized Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
V21BBa_K382050Arabidopsis-optimized MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencekanpatlink
V22BBa_K382051Arabidopsis-optimized MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencekannptIIlink
V25BBa_K382052Arabidopsis-optimized BrazzeinSweet-tasting protein. 54 amino acid sequence. kanpatlink
V26BBa_K382053Arabidopsis-optimized BrazzeinSweet-tasting protein. 54 amino acid sequence. kannptIIlink
V29BBa_K382054amiRNA GFPamiRNA construct for GFP knockdownkanpatlink
V30BBa_K382055amiRNA GFPamiRNA construct for GFP knockdownkannptIIlink
V33BBa_K382058amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownkanpatlink
V35BBa_K382059amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownkanpatlink
V37BBa_K382060amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownkanpatlink
V34BBa_K382061amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownkannptIIlink
V36BBa_K382062amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownkannptIIlink
V38BBa_K382063amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownkannptIIlink
BBa_K382064amiRNA LTP LTP knockdown constructkanpat link
BBa_K382065amiRNA LTP LTP knockdown constructkannptII link
BBa_K382066ihpRNA Ger Intron hairpin Ger Knockdown constructkanpatlink
BBa_K382067ihpRNA Ger Intron hairpin Ger Knockdown constructkannptIIlink
BBa_K382068ihpRNA BetV1Intron hairpin Bet Knockdown constructkanpatlink
BBa_K382069ihpRNA BetV1Intron hairpin Bet Knockdown constructkannptIIlink
BBa_K382070ihpRNA LTPIntron hairpin LTP Knockdown constructkanpatlink
BBa_K382071ihpRNA LTPIntron hairpin LTP Knockdown constructkannptIIlink

Retrieved from "http://2010.igem.org/Team:Harvard/parts"