Team:NYMU-Taipei

SpeedyBac

• Goal:
  

Provide a faster assay system for exploring the design rules of synthetic biology.

• Why do we want to do that?
  

There are already many genetic parts in the Biobrick Parts Registry and the numbers are growing rapidly. Every year every igem teams will build one or more circuits based on the parts at partsregistry. But where are the design rules to put these parts into circuits of devices and systems? Apparently, the "Assembly Standards" listed at the partsregistry are only used to connect compatible restriction enzyme cutting sites. They are NOT designing principles. Our iGEM team is very interested in the detailed design rules played in the central dogma; especially those principles connect mRNA translation to protein folding. Traditionally, we know about the circuits we made are working or not by the expression of reporter genes. But now we want to quantitative description of gene expression in both space and time. For the above reasons, we must to be speed up the experiment for researching the more rules.

• Specific aims:
  
  • Quantitative description of gene expression in both space and time.
  • Specific insight into the flow of genetic information.
  • Speedy ways to report and stop gene expression.
• Our design:
  

To achieve our specific aim, we have designed a novel reporting assay (Speedy reporter) for quickly detect and measure the mRNA location and quantity, it can be also use for protein detection. And we design a novel switch (Speedy switch) for control the translation in gene expression. We have also designed a faster degradation system (Speedy protein degrader); it allows us to regulate the degradation time for study the mRNA without the interference from translation and quickly stop the gene expression.
The parts our project is made up of:

• Speedy switch
  • Faster production of protein by inducing the translation of pre-transcribed RNA molecules.
• Speedy reporter
  • Using mRNA aptamers and split GFP-eIF4A reporter systems to show promoter activity faster.
• Speedy protein degrader
  • Fast, specific, and constitutive proteolysis achieved by engineering fluorescent proteins with LVA

• Our design
  

To achieve our specific aim, we have designed a novel reporting device (Speedy reporter) for quickly detectin and measuring the mRNA location and quantity, it can also be used for protein detection. And we design a novel switch (Speedy switch) for control the mRNA translation of gene expression. We have also designed a faster degradation device (Speedy protein degrader); it allows us to regulate the degradation time for studying the mRNAs without the interference from translation and quickly stopping the gene expression.

Our SpeedyBac system is made up of the following three devices:

• Speedy switch
  • Faster production of protein by inducing the translation of pre-existing mRNA molecules.
• Speedy reporter
  • Using mRNA aptamers and split GFP-eIF4A reporter designs to detect promoter activity faster.
• Speedy protein degrader
  • Fast, specific, and constitutive proteolysis achieved by engineering fluorescent proteins tagged with LVA

• Our institute

The official web pages of our school - National Yang Ming University (NYMU):

• [http://web.ym.edu.tw/front/bin/home.phtml in Chinese]
• [http://nymu-e.web.ym.edu.tw/front/bin/home.phtml in English]

Click the following two links to see The Beauty of NYMU

• [http://issue.ym.edu.tw/cia/new/ Take a panoramic scenery view of our university]
• [http://issue.ym.edu.tw/cia/new/tw/ym720.html Take a tour of our university]