Team:Harvard/parts

From 2010.igem.org

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<tr><td>19</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator.  Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr>
<tr><td>19</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator.  Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr>
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<tr><td>20</td><td>BBa_K382025</td><td><i>Arabidopsis</i>-optimized Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr>
+
<tr><td>20</td><td>BBa_K382025</td><td><i>Arabidopsis</i>-optimized Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on N-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr>
<tr><td>21</td><td>BBa_K382026</td><td><i>Arabidopsis</i>-optimized Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on N-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr>
<tr><td>21</td><td>BBa_K382026</td><td><i>Arabidopsis</i>-optimized Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on N-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr>
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<tr><td>22</td><td>BBa_K382027</td><td><i>Arabidopsis</i>-optimized Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on N-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr>
+
<tr><td>22</td><td>BBa_K382027</td><td><i>Arabidopsis</i>-optimized Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr>
<tr><td>23</td><td>BBa_K382028</td><td><i>Arabidopsis</i>-optimized Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr>
<tr><td>23</td><td>BBa_K382028</td><td><i>Arabidopsis</i>-optimized Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr>

Revision as of 17:36, 27 October 2010




parts

biobrick nameoriginal namedescriptionresistance (bacteria)resistance (plants)sequence
1BBa_K382000pORE Open Series Vector with BioBrick SitesAgrobacterium vector open series with biobrick cloning sitekanpatlink
2BBa_K382001pORE Open Series Vector with BioBrick SitesAgrobacterium vector open series with biobrick cloning sitekannptIIlink
3BBa_K382002pORE Expression Series Vector with BioBrick SitesAgrobacterium vector with ENTCUP2 promoter and biobrick cloning sitekanpatlink
4BBa_K382003pORE Expression Series Vector with BioBrick SitesAgrobacterium vector with ENTCUP2 promoter and biobrick cloning sitekannptIIlink
5BBa_K382004pORE Reporter Series Vector with BioBrick SitesAgrobacterium vector with gusA reporter and biobrick cloning sitekannptIIlink
6BBa_K382005pORE Reporter Series Vector with BioBrick SitesAgrobacterium vector with smgfp reporter and biobrick cloning sitekannptIIlink
7BBa_K382010amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownchloramphenicol-link
8BBa_K382011amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-link
9BBa_K382012amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-link
10BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructamp-link
11BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-link
12BBa_K382017amiRNA LTP LTP knockdown constructamp- link
13BBa_K382018PDK intron hairpinIntron hairpin constructamp-link
14BBa_K382019ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-link
15BBa_K382020Arabidopsis-optimized BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-link
16BBa_K382021Arabidopsis-optimized MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-link
17BBa_K382022pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-link
18BBa_K382023NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-link
19BBa_K382024NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on 5' end.chloramphenicol-link
20BBa_K382025Arabidopsis-optimized Miraculin StrepII N-terminusMiraculin with StrepII tag on N-terminus for Western Blot.chloramphenicol-link
21BBa_K382026Arabidopsis-optimized Brazzein StrepII N-terminusBrazzein with StrepII tag on N-terminus for Western Blot.chloramphenicol-link
22BBa_K382027Arabidopsis-optimized Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
23BBa_K382028Arabidopsis-optimized Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
24BBa_K382030RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.chloramphenicol-link
25BBa_K382031RXRLc Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozideamp-link
26BBa_K382033Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide chloramphenicol-link
27BBa_K382034Act2pLacOActin promoter with LacO sites amp-link
28BBa_K382035LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-link
29BBa_K382036RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.amp-link
30BBa_K382037Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide amp-link
31BBa_K382040Arabidopsis-optimized Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
32BBa_K382041Arabidopsis-optimized Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
33BBa_K382050Arabidopsis-optimized MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencekanpatlink
34BBa_K382051Arabidopsis-optimized MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencekannptIIlink
35BBa_K382052Arabidopsis-optimized BrazzeinSweet-tasting protein. 54 amino acid sequence. kanpatlink
36BBa_K382053Arabidopsis-optimized BrazzeinSweet-tasting protein. 54 amino acid sequence. kannptIIlink
37BBa_K382054amiRNA GFPamiRNA construct for GFP knockdownkanpatlink
38BBa_K382055amiRNA GFPamiRNA construct for GFP knockdownkannptIIlink
39BBa_K382058amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownkanpatlink
40BBa_K382059amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownkanpatlink
41BBa_K382060amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownkanpatlink
42BBa_K382061amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownkannptIIlink
43BBa_K382062amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownkannptIIlink
44BBa_K382063amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownkannptIIlink
45BBa_K382064amiRNA LTP LTP knockdown constructkanpat link
46BBa_K382065amiRNA LTP LTP knockdown constructkannptII link
47BBa_K382066ihpRNA Ger Intron hairpin Ger Knockdown constructkanpatlink
48BBa_K382067ihpRNA Ger Intron hairpin Ger Knockdown constructkannptIIlink
49BBa_K382068ihpRNA BetV1Intron hairpin Bet Knockdown constructkanpatlink
50BBa_K382069ihpRNA BetV1Intron hairpin Bet Knockdown constructkannptIIlink
51BBa_K382070ihpRNA LTPIntron hairpin LTP Knockdown constructkanpatlink
52BBa_K382071ihpRNA LTPIntron hairpin LTP Knockdown constructkannptIIlink

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