Achievements

Bronze medal requirements

Register the team

Successfully complete and submit a project summary form

Create a Wiki which includes all the details of the project

Present a presentation and a poster at the iGEM Jamboree

Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts

Data was entered for 11 new biobrick parts

Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts

We designed and submitted DNA for three new biobrick parts

Cytoplasmic maltose binding protein (malESS)

IbpAb/ fxsA fusion promoter - cpxP Promoter We also built the following new constructs: -ibpAB reporter CpxR reporter DegP reporter MalE generator MalE31 generator K13500-I13507-I0500-B0034-malE31 All of these parts were verified via resriction digests and PCR, and then finally sequenced. Silver medal Requirments Demonstrate that one of your parts works as expected Through our characeroization data, we showed that several of our parts worked as expected. The cpxR reporter was found to report on misfolding protein. We characterized this promoter with varying concentrations of folding and midfolding proteins produced. We also tested this promoter with NLPE, an outer membrane lipoprotein that literature has found activates the cpx pathway. Finally, we tested this promoter in varying heat shock conditions. Both MalE and malE31 also worked as expected, malE folding in the periplams and malE31 showing a misfolding reponse. This was indictaed through tetsing with a reporter from another lab (Raivio) (for more info click here) as well as tetsing with our cpxR reporter click here. We characetrized the cpxR reporter, malE31 ibpAB and . See characterization data here. Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry. -we entered new DNA as well as new sequences for malE and malE31. - we entered characterization data for the cpxR promoter Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system. -We tetsed out a part for Lethbridge. We characterized it for possible misfodling in the cytoplasm. Resuylts can be found here. - We also attended a regional workship as well as a mock Igem event with the Univeristy of Salberta as well as the Univeristy of Lethbridge. Here we helped each their out with our projects by practicing our presentaitons for each other, giving suggestions, future directions as well as touble shooting -Finally our team filled out surveys for the following teams: - This year we chose to approach ethjcs by making a pods acts covering a few different ethical issues pertaining to synthetic biology. We felt tat this would be a novel way to increase awareness about the field of syntyehtic biology while having a discussion of important issues. We felt that this would be a much more accessible way for the public to gain a better understanding of what the real fears in sythetic biology is as well as to dispell some of the misleading iompressions given by media sources.