"
Team:Tsinghua/Notebook/8 October 2010
From 2010.igem.org
(Difference between revisions)
Shelly (Talk | contribs)
(New page: == Module I, Group 2c == Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I. ---- Double digestion system eGFP 5ul EcoR I 1ul Pst I ...)
Newer edit →
(New page: == Module I, Group 2c == Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I. ---- Double digestion system eGFP 5ul EcoR I 1ul Pst I ...)
Newer edit →
Revision as of 13:39, 27 October 2010
Module I, Group 2c
Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I.
Double digestion system
eGFP 5ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 11ul
mCherry 4ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 12ul ---- Kan 4ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 12ul
Incubate at 37 centidegree overnight.
