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Team:TU Delft/24 September 2010 content
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Today we did a DLT to make 014 (011+028=>alkB2 gene cluster), 022 (017+026=>ladA, ADH and ALDH) and 023 (027+026, same as 022 but with different promoter). The ligation mixes were used to transform commercial TOP10 cells. | Today we did a DLT to make 014 (011+028=>alkB2 gene cluster), 022 (017+026=>ladA, ADH and ALDH) and 023 (027+026, same as 022 but with different promoter). The ligation mixes were used to transform commercial TOP10 cells. | ||
| + | No encouraging results from the resting cell assays :-( We are going to use other protocol. Maybe cell disruption and NADH measurements... | ||
=Salt tolerance= | =Salt tolerance= | ||
We ran an innitial test of our salt tolerance biobrick. | We ran an innitial test of our salt tolerance biobrick. | ||
We'll have the results on monday | We'll have the results on monday | ||
Latest revision as of 13:22, 27 October 2010
Alkane degradation
Today we did a DLT to make 014 (011+028=>alkB2 gene cluster), 022 (017+026=>ladA, ADH and ALDH) and 023 (027+026, same as 022 but with different promoter). The ligation mixes were used to transform commercial TOP10 cells.
No encouraging results from the resting cell assays :-( We are going to use other protocol. Maybe cell disruption and NADH measurements...
Salt tolerance
We ran an innitial test of our salt tolerance biobrick. We'll have the results on monday
