Team:UNIPV-Pavia/Calendar/July/settimana2

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We checked the presence of colonies in plates.
We checked the presence of colonies in plates.
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All plates showed colonies!
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All plates showed red colonies!
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Revision as of 13:03, 9 July 2010
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JULY: WEEK 2



July, 5th

July, 6th

July, 7th

July, 8th

Transformation of 1ul (~4ng) of miniprepped <partinfo>BBa_J23118</partinfo> into 100ul of home-made competent cells

  • MG1655
  • BW25141
  • BW25142
  • BW53474
  • DH5-alpha (as control)

to verify the transformation efficiency.

Transformed bacteria were plated on LB+Amp and left overnight in oven, 37°C.

July, 9th

We checked the presence of colonies in plates. All plates showed red colonies!

MG1655 plate
BW25141 plate
BW25142 plate
BW53474 plate
DH5-alpha plate

We calculated (thanks Nicolò) efficiency as #colonies/ug DNA plated

Strain #colonies Efficiency
MG1655 379 ~10^5
BW25141 1431 ~10^6
BW25142 2374 ~10^6
BW53474 9984 ~10^7
(very difficult to count correctly)
DH5alpha 7475
(only 100ul of cells plated) 		~ 10^7
(very difficult to count correctly,
previous tests showed an efficiency of ~10^8)

Calendar

July

week 1

week 2

week 3

week 4


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