Team:Yale/Our Project/Protocols/standard curve

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1. Make serial 10-1 dilutions of 1mM to 10pM CuSO4 in LB media.<br/>
1. Make serial 10-1 dilutions of 1mM to 10pM CuSO4 in LB media.<br/>
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2. Dissolve Bathocuproinedisulfonic in x amount of water for a final concentration of  x M solution. <br/>
+
2. Make 100mM solution of  Bathocuproinedisulfonic (BCS) reagent. <br/>
3. Measure absorbance (483nm) of LB media without copper as blank. <br/>
3. Measure absorbance (483nm) of LB media without copper as blank. <br/>
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4. Add 10µL of 100mM ascorbate to 1mL of standard copper solution and 10µL of the reagent. <br/>
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4. Add 1µL of 1M ascorbate and and 1µL of BCS to 100µL of standard copper solution. <br/>
5. Measure absorbance of each copper solution at (483nm) <br/>
5. Measure absorbance of each copper solution at (483nm) <br/>
6. Record results.<br/>
6. Record results.<br/>

Latest revision as of 12:24, 27 October 2010

iGEM Yale

protocols

Copper-Bathocuproinedisulfonic acid standard absorbance curve measurement

1. Make serial 10-1 dilutions of 1mM to 10pM CuSO4 in LB media.
2. Make 100mM solution of Bathocuproinedisulfonic (BCS) reagent.
3. Measure absorbance (483nm) of LB media without copper as blank.
4. Add 1µL of 1M ascorbate and and 1µL of BCS to 100µL of standard copper solution.
5. Measure absorbance of each copper solution at (483nm)
6. Record results.

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