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Team:Yale/MeetingNotes
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Dspakowicz (Talk | contribs) (→July 8) |
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*Pick a concentration from the cell growth assay, like 1 mM Cu? | *Pick a concentration from the cell growth assay, like 1 mM Cu? | ||
*Grow shaking, at time points spin out the cells and measure the absorbance | *Grow shaking, at time points spin out the cells and measure the absorbance | ||
| - | **assaying for loss of copper | + | **this is assaying for loss of copper |
3. Does phsABC confer copper resistance? | 3. Does phsABC confer copper resistance? | ||
| - | 96-well plate assay | + | *96-well plate assay |
| - | same as before, but with the phsABC plasmid | + | *same as before, but with the phsABC plasmid |
| - | + | **Since Leah has this assay dialed-in, this is a great job for a part-time helper to run and analyze! | |
4. Cu resistance | 4. Cu resistance | ||
| - | shipping in a day or two | + | * will be shipping in a day or two |
| - | pRJ1004 (Cu efflux protein) (Jon | + | **pRJ1004 (Cu efflux protein) (Jon will email to follow up) |
| - | massive plasmid, no antibiotic resistance | + | ***massive plasmid, no antibiotic resistance |
| - | Get together next week to request several plasmids | + | **Get together next week to request several plasmids (Tuesday morning at 10 am). |
| - | + | ||
5. Email Kara about the mold (Jon) | 5. Email Kara about the mold (Jon) | ||
6. Ways to get students involved (Dan email the group) | 6. Ways to get students involved (Dan email the group) | ||
| - | Ask when they're available, delegate a task | + | *Ask when they're available, delegate a task |
| - | make sure someone is there to help them | + | *make sure someone is there to help them |
Revision as of 21:12, 8 July 2010
July 8
1. Cloning issues: Did the double digestion of the terminator work?
- The EcoRI was old and might have failed
- SpeI not very efficient in EcoRI buffer, will try to incubate longer
- The ligation didn't work, but there are lots of ways to keep trying. This is a great way for people to come help during the week.
2.Copper precipitation assay
- Pick a concentration from the cell growth assay, like 1 mM Cu?
- Grow shaking, at time points spin out the cells and measure the absorbance
- this is assaying for loss of copper
3. Does phsABC confer copper resistance?
- 96-well plate assay
- same as before, but with the phsABC plasmid
- Since Leah has this assay dialed-in, this is a great job for a part-time helper to run and analyze!
4. Cu resistance
- will be shipping in a day or two
**pRJ1004 (Cu efflux protein) (Jon will email to follow up) ***massive plasmid, no antibiotic resistance **Get together next week to request several plasmids (Tuesday morning at 10 am).
5. Email Kara about the mold (Jon)
6. Ways to get students involved (Dan email the group) *Ask when they're available, delegate a task *make sure someone is there to help them
