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Revision as of 21:07, 8 July 2010 (view source) Dspakowicz (Talk | contribs) (New page: ==July 8==) ← Older edit		Revision as of 21:08, 8 July 2010 (view source) Dspakowicz (Talk | contribs) (→July 8) Newer edit →
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	==July 8==		==July 8==
		+
		+	1. Cloning issues:
		+	Did the double digestion of the terminator work?
		+	EcoRI might have failed
		+	SpeI not very efficient in EcoRI buffer, run longer
		+	ligation didn't work, keep trying
		+
		+
		+	2.Copper precipitation assay
		+	Pick a concentration from the cell growth assay
		+	1 mM Cu?
		+	Grow shaking, at time points spin out the cells and measure the absorbance
		+	assaying for loss of copper
		+
		+
		+	3. Does phsABC confer copper resistance?
		+	96-well plate assay
		+	same as before, but with the phsABC plasmid
		+
		+
		+	4.  Cu resistance
		+	shipping in a day or two
		+	pRJ1004 (Cu efflux protein) (Jon) will email
		+	massive plasmid, no antibiotic resistance
		+	Get together next week to request several plasmids
		+	Tuesday morning at 10 am.
		+
		+	5. Email Kara about the mold (Jon)
		+
		+	6. Ways to get students involved (Dan email the group)
		+	Ask when they're available, delegate a task
		+	make sure someone is there to help them

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Revision as of 21:08, 8 July 2010

July 8

1. Cloning issues: Did the double digestion of the terminator work? EcoRI might have failed SpeI not very efficient in EcoRI buffer, run longer ligation didn't work, keep trying

2.Copper precipitation assay Pick a concentration from the cell growth assay 1 mM Cu? Grow shaking, at time points spin out the cells and measure the absorbance assaying for loss of copper

3. Does phsABC confer copper resistance? 96-well plate assay same as before, but with the phsABC plasmid

4. Cu resistance shipping in a day or two pRJ1004 (Cu efflux protein) (Jon) will email massive plasmid, no antibiotic resistance Get together next week to request several plasmids Tuesday morning at 10 am.

5. Email Kara about the mold (Jon)

6. Ways to get students involved (Dan email the group) Ask when they're available, delegate a task make sure someone is there to help them