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Team:DTU-Denmark/Parts
From 2010.igem.org
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<h1>BioBricks submitted</h1> | <h1>BioBricks submitted</h1> | ||
<p align="justify">BBa_K374008 | <p align="justify">BBa_K374008 | ||
| - | <p align="justify">Divergent promoters from Gifsy1 phage and | + | <p align="justify">Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR</p> |
<p align="justify">Expression of any gene located upstream of this BioBrick will not be expressed. However, any gene located downstream will be expressed. | <p align="justify">Expression of any gene located upstream of this BioBrick will not be expressed. However, any gene located downstream will be expressed. | ||
Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p> | Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p> | ||
Reference | Reference | ||
<p align="justify">BBa_K374009 | <p align="justify">BBa_K374009 | ||
| - | <p align="justify">Divergent promoters from Gifsy2 phage and | + | <p align="justify">Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR</p> |
| - | + | <p align="justify">Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p> | |
</p> | </p> | ||
</td> | </td> | ||
Revision as of 08:38, 27 October 2010
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BioBricks submittedBBa_K374008 Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR Expression of any gene located upstream of this BioBrick will not be expressed. However, any gene located downstream will be expressed. Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli. ReferenceBBa_K374009 Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli. |
Parts
New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
<groupparts>iGEM010 DTU-Denmark</groupparts>
