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Team:Queens-Canada/3 August 2010
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(New page: <h2>August 3, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Steve and Chris '''unc-54 3'UTR'''::1A3, '''pRab-7'''::1A3, '...)
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(New page: <h2>August 3, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Steve and Chris '''unc-54 3'UTR'''::1A3, '''pRab-7'''::1A3, '...)
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Revision as of 06:00, 27 October 2010
August 3, 2010
Liquid Culture
Steve and Chris
unc-54 3'UTR::1A3, pRab-7::1A3, pMec-7::1A3
Digestion-Ligation
Thai and Hao
mCherry, pCeh-12, pSrb-6, pOdr-1, pOdr-10, NpHR, eYFP, pHsp-3, pStr-220, pFlp-1, pOsm-10, pStr-1, eCFP, pSB1C3
Cut with EcoRI and PstI into pSB1C3. Backbone treated with CIAP. Used T4 ligase.
Heat Shock
mCherry, pCeh-12, pSrb-6, pOdr-1, pOdr-10, NpHR, eYFP, pHsp-3, pStr-220, pFlp-1, pOsm-10, pStr-1, eCFP, pSB1C3
Transformed TOP10 using previous ligation products.
PCR
Chris
pFlp-1, pHsp-3, NpHR
Miniprep
Chris and Mary and Louis
Solution II used in place of Solution I.
