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Team:Queens-Canada/22 July 2010
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(New page: <h2>July 22, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Gel Electrophoresis</h3> Steve '''eYFP''' Extract Used 0.7 g agarose in 100 m...)
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(New page: <h2>July 22, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Gel Electrophoresis</h3> Steve '''eYFP''' Extract Used 0.7 g agarose in 100 m...)
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Revision as of 05:54, 27 October 2010
July 22, 2010
Gel Electrophoresis
Steve
eYFP Extract
Used 0.7 g agarose in 100 mL TBE. Gel run for 50 mins at 100 V.
Miniprep
Hao and Thai PRF4
Sequencing Prep
Chris and Thai
pSip-1::1A3
Used 4 µL of 2µM primers with 8 µL of plasmid.
PCR
Yuli
pSip-1:::eGFP, pRab-7:::eGFP, pMec-7:::eGFP, unc-54 3'UTR, pStr-1
Program run using 9:1 FAST:HIFI blend with an extension time of 45 s and annealing temperature of 61 °C.
Gel Electrophoresis
Chris
pSip-1:::eGFP, pRab-7:::eGFP, pMec-7:::eGFP, unc-54 3'UTR, pStr-1
Used 0.3 g agarose in 50 mL TBE. Gel run for 45 mins at 100 V.
PCR
Steve
pSB1A3
Program run using 9:1 FAST:HIFI blend with an extension time of 70 s and annealing temperature of 62 °C.
PCR
Yuli
eYFP1a and eYFP2a
Program run using 9:1 FAST:HIFI blend with an extension time of 45 s and annealing temperature of 58 °C.
