Team:Queens-Canada/29 June 2010

Liquid Culture

Thai and Hao

BBa_I13522(Amp)

Incubated at 37 °C starting at 11:00.

Gel Electrophoresis

Thai and Hao

pMec-7, pRab-7, pSip-1

PCR

Basia and Thai

pIC576-1a, pIC57-1b, pIC518-1a, pIC518-b.

Program run using HIFI with an extension time of 30 s and annealing temperature of 61 °C.

PCR

Yuli and Steve

pSip-1:::eGFP extract, pRab-7:::eGFP extract, BBa_I1408

Program run using HIFI with an extension time of 165 s and annealing temperature of 58 °C.

PCR

Thai NpHR, ChR2

Program run using HIFI with an extension time of 30 s and annealing temperature of 60 °C.

PCR

Hao unc-22 C, unc-22 A

Program run using HIFI with an extension time of 32 s and annealing temperature of 58 °C.

Gel Electrophoresis

Hao

pIC576, pIC518, NpHR, ChR2, unc-22 C, unc-22 A

Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.