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Team:Queens-Canada/16 June 2010
From 2010.igem.org
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<h2>June 16, 2010</h2> | <h2>June 16, 2010</h2> | ||
<h3>[[Team:Queens-Canada/protocols#PCR Purification|PCR Purification]]</h3> | <h3>[[Team:Queens-Canada/protocols#PCR Purification|PCR Purification]]</h3> | ||
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Program run (without primers) using TAQ with an extension time of 30 s and annealing temperature of 57 °C for 5 cycles. | Program run (without primers) using TAQ with an extension time of 30 s and annealing temperature of 57 °C for 5 cycles. | ||
Primers were then added and program was run with annealing temperature of 60 °C. | Primers were then added and program was run with annealing temperature of 60 °C. | ||
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Latest revision as of 05:21, 27 October 2010
June 16, 2010
PCR Purification
Hao and Chris
pHsp-3, pSip-1, pMec-7, pRab-7, pUnc-47, BBa_I714891
Products stored in -20 °C freezer.
PCR Stitching
Hao and Chris
pHsp-3, pRab-7, pSip-1, pUnc-47, pMec-7 with BBa_I714891
Program run (without primers) using TAQ with an extension time of 30 s and annealing temperature of 57 °C for 5 cycles. Primers were then added and program was run with annealing temperature of 60 °C.
