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Week of 10/18
From 2010.igem.org
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| + | ==October 19th== | ||
| + | |||
| + | *Minipreps: | ||
| + | -J23116+K091146 | ||
| + | -A3 | ||
| + | |||
| + | There had been previous minipreps completed; however, the two above did not turn out correctly the first time. Because of this problem, they were miniprepped once more. | ||
| + | |||
| + | *Digested: | ||
| + | -K091107+J23116 (EX) | ||
| + | -K091107+J23108 (EX) | ||
| + | -K091146+J23108 (EX) | ||
| + | -K091146+J13116 (EX) | ||
| + | -AFP (EX) | ||
| + | -TetCIR (EX) | ||
| + | -LTC+J23116 (ES) | ||
| + | -LTC+J23108 (ES) | ||
| + | |||
| + | Note: There was protein contamination in K091146+J23116 after the digest. | ||
| + | |||
| + | *Gel Order: | ||
| + | -Ladder | ||
| + | -K091107+J23116 | ||
| + | -K091107+J23108 | ||
| + | -K091146+J23108 | ||
| + | -AFP | ||
| + | -TetCIR | ||
| + | -LTC+J23116 | ||
| + | -LTC+J23108 | ||
| + | -K091146+J13116 | ||
| + | |||
[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | ||
Revision as of 04:59, 27 October 2010
October 19th
- Minipreps:
-J23116+K091146 -A3
There had been previous minipreps completed; however, the two above did not turn out correctly the first time. Because of this problem, they were miniprepped once more.
- Digested:
-K091107+J23116 (EX) -K091107+J23108 (EX) -K091146+J23108 (EX) -K091146+J13116 (EX) -AFP (EX) -TetCIR (EX) -LTC+J23116 (ES) -LTC+J23108 (ES)
Note: There was protein contamination in K091146+J23116 after the digest.
- Gel Order:
-Ladder -K091107+J23116 -K091107+J23108 -K091146+J23108 -AFP -TetCIR -LTC+J23116 -LTC+J23108 -K091146+J13116
