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Week of 10/4
From 2010.igem.org
(Difference between revisions)
(→October 6th) |
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*Gel Order: | *Gel Order: | ||
| + | - 1.a,b,c | ||
| + | - 2.a,b,c | ||
| + | - 3.a,b,c | ||
| + | - 4.a,b,c | ||
| + | - 5.a,b,c | ||
| + | - 6.a,b,c | ||
| + | |||
| + | *Grown In Flasks: | ||
| + | -1a (R1062+RFP+J23100+LuxR) | ||
| + | -2a (R1062+RFP+J23108+LuxR) | ||
| + | -3a (R1062+RFP+J23116+LuxR) | ||
| + | -4a (R0063+RFP+J23100+LuxR) | ||
| + | -5a (R0063+RFP+J23108+LuxR) | ||
| + | -6a (R0063+RFP+J23116+LuxR) | ||
| + | |||
[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | ||
Revision as of 04:51, 27 October 2010
October 5th
- Digests:
-R1062+RFP (SP) -R0061+RFP (SP)
- Ligations:
-R1062+RFP -->J23100+LuxR (B)
-->J23108+LuxR (B)
-->J23116+LuxR (B)
-R0063+RFP -->J23100+LuxR (B)
-->J23108+LuxR (B)
-->J23116+LuxR (B)
October 6th
- Colony PCR:
1. R1062+RFP + J23100+LuxR 2. R1062+RFP + J23108+LuxR 3. R1062+RFP + J23116+LuxR 4. R0063+RFP + J23100+LuxR 5. R0063+RFP + J23108+LuxR 6. R0063+RFP + J23116+LuxR
They were then run on a gel after being PCRd.
- Gel Order:
- 1.a,b,c - 2.a,b,c - 3.a,b,c - 4.a,b,c - 5.a,b,c - 6.a,b,c
- Grown In Flasks:
-1a (R1062+RFP+J23100+LuxR) -2a (R1062+RFP+J23108+LuxR) -3a (R1062+RFP+J23116+LuxR) -4a (R0063+RFP+J23100+LuxR) -5a (R0063+RFP+J23108+LuxR) -6a (R0063+RFP+J23116+LuxR)
