Team:Purdue/MS media preparation

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(Difference between revisions)

Revision as of 14:38, 8 July 2010

For one liter of 0.5 [http://en.wikipedia.org/wiki/Murashige_and_Skoog_medium MS]:

2.165 g MS salt
15 g sucrose (1.5%)
12 g agar (1.2%)
1 N NaOH or KOH

Procedure

Use container that is twice as large as solution volume (ex. for 1 L solution one should use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions. Finally, add agar. There is no need to dissolve the agar prior to autoclaving.

Autoclave Notes

Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.

@@ Line 1: / Line 1: @@
-[[Arabidopsis grown on 0.5 MS media]]
+For one liter of 0.5 [http://en.wikipedia.org/wiki/Murashige_and_Skoog_medium MS]:
-For one liter of 0.5 MS:
+*2.165 g MS salt
+*15 g sucrose (1.5%)
+*12 g agar (1.2%)
+*1 N NaOH or KOH
-.165 gm MS salt + 15 gm sucrose (1.5%) + 12 gm agar (1.2%). Adjust the pH to 5.7 with I N NaOH.
+==Procedure==
-[[Procedure]]
+Use container that is twice as large as solution volume (ex. for 1 L solution one should use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions.  Finally, add agar.  There is no need to dissolve the agar prior to autoclaving.
-Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7. LASTLY, add agar.
+==Autoclave Notes==
-[[AutoClave Notes:]]
+Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.
-Use container that is twice as large as solution volume (ex. for 500 mL solution one should use 1000mL flask). Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.