Team:Purdue/MS media preparation

From 2010.igem.org

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[[Arabidopsis grown on 0.5 MS media]]
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For one liter of 0.5 [http://en.wikipedia.org/wiki/Murashige_and_Skoog_medium MS]:
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For one liter of 0.5 MS:
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*2.165 g MS salt
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*15 g sucrose (1.5%)
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*12 g agar (1.2%)
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*1 N NaOH or KOH
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2.165 gm MS salt + 15 gm sucrose (1.5%) + 12 gm agar (1.2%). Adjust the pH to 5.7 with I N NaOH.
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==Procedure==
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[[Procedure]]
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Use container that is twice as large as solution volume (ex. for 1 L solution one should use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions.  Finally, add agar.  There is no need to dissolve the agar prior to autoclaving.
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Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7. LASTLY, add agar.
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==Autoclave Notes==
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[[AutoClave Notes:]]
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Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.
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Use container that is twice as large as solution volume (ex. for 500 mL solution one should use 1000mL flask). Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.
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Revision as of 14:38, 8 July 2010

For one liter of 0.5 [http://en.wikipedia.org/wiki/Murashige_and_Skoog_medium MS]:

  • 2.165 g MS salt
  • 15 g sucrose (1.5%)
  • 12 g agar (1.2%)
  • 1 N NaOH or KOH

Procedure

Use container that is twice as large as solution volume (ex. for 1 L solution one should use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions. Finally, add agar. There is no need to dissolve the agar prior to autoclaving.

Autoclave Notes

Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.