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Team:Purdue/MS media preparation
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(New page: Arabidopsis grown on 0.5 MS media For one liter of 0.5 MS: 2.165 gm MS salt + 15 gm sucrose (1.5%) + 12 gm agar (1.2%). Adjust the pH to 5.7 with I N NaOH. Procedure Add MS sal...)
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(New page: Arabidopsis grown on 0.5 MS media For one liter of 0.5 MS: 2.165 gm MS salt + 15 gm sucrose (1.5%) + 12 gm agar (1.2%). Adjust the pH to 5.7 with I N NaOH. Procedure Add MS sal...)
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Revision as of 14:25, 8 July 2010
Arabidopsis grown on 0.5 MS media
For one liter of 0.5 MS:
2.165 gm MS salt + 15 gm sucrose (1.5%) + 12 gm agar (1.2%). Adjust the pH to 5.7 with I N NaOH.
Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7. LASTLY, add agar.
AutoClave Notes:
Use container that is twice as large as solution volume (ex. for 500 mL solution one should use 1000mL flask). Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.
