"
Team:Berkeley/Project/Other organisms
From 2010.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
* The target organism's phagocytic vesicles must contain cholesterols susceptible to our [https://2010.igem.org/Team:Berkeley/Project/Vesicle_Buster Vesicle-Buster] device. | * The target organism's phagocytic vesicles must contain cholesterols susceptible to our [https://2010.igem.org/Team:Berkeley/Project/Vesicle_Buster Vesicle-Buster] device. | ||
* The target organism must be able to survive in media that is compatible with our [https://2010.igem.org/Team:Berkeley/Project/Self_Lysis Self-Lysis] device. | * The target organism must be able to survive in media that is compatible with our [https://2010.igem.org/Team:Berkeley/Project/Self_Lysis Self-Lysis] device. | ||
| - | We have started looking at other organisms to deliver payloads to using our payload delivery device. Having screened about a dozen single-celled, freshwater eukaryotes for phagocytosis of <i>E. coli</i> and ability to survive in various concentrations of LB, we are now focusing our efforts on [http://en.wikipedia.org/wiki/Tetrahymena Tetrahymena], [http://en.wikipedia.org/wiki/ | + | We have started looking at other organisms to deliver payloads to using our payload delivery device. Having screened about a dozen single-celled, freshwater eukaryotes for phagocytosis of <i>E. coli</i> and ability to survive in various concentrations of LB, we are now focusing our efforts on [http://en.wikipedia.org/wiki/Tetrahymena Tetrahymena], [http://en.wikipedia.org/wiki/Blepharisma Blepharisma] and [http://en.wikipedia.org/wiki/Paramecium Paramecia]. We have already determined that these three types of organisms will readily eat <i>E. coli</i> and are capable of surviving in mixtures of their culturing media and LB. |
{| cellpadding="10" cellspacing="10" | {| cellpadding="10" cellspacing="10" | ||
|Tetrahymena | |Tetrahymena | ||
| Line 16: | Line 16: | ||
||[[Image:Tetra-GFP.jpg|thumb|300px|Tetrahymena: GFP only]] | ||[[Image:Tetra-GFP.jpg|thumb|300px|Tetrahymena: GFP only]] | ||
|- | |- | ||
| - | | | + | |Blepharisma |
| - | ||[[Image: | + | ||[[Image:Blepharisma-Phase.jpg|thumb|300px|Blepharisma: Phase contrast]] |
| - | ||[[Image: | + | ||[[Image:Blepharisma-Phase+GFP.jpg|thumb|300px|Blepharisma: Phase contrast and GFP]] |
| - | ||[[Image: | + | ||[[Image:Blepharisma-GFP.jpg|thumb|300px|Blepharisma: GFP only]] |
|- | |- | ||
|Paramecia | |Paramecia | ||
Revision as of 02:57, 27 October 2010
- Home
- Project
- Overview
- Motivation
- Other Organisms
- Parts
- Self-Lysis
- Vesicle-Buster
- Payload
- [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Berkeley Parts Submitted]
- Results
- Judging
- Clotho
- Human Practices
- Team Resources
- Who We Are
- Notebooks:
- [http://www.openwetware.org/wiki/Berk2010-Daniela Daniela's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Christoph Christoph's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Amy Amy's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Tahoura Tahoura's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Conor Conor's Notebook]
Our project focused on the delivery of payloads to the cytoplasms of choanoflagellates, but its simple design makes it applicable to a host of other eukaryotes. Our design is only limited in three main aspects as to its potential targets:
- The target organism must be able to naturally phagocytose Escheria Coli.
- The target organism's phagocytic vesicles must contain cholesterols susceptible to our Vesicle-Buster device.
- The target organism must be able to survive in media that is compatible with our Self-Lysis device.
We have started looking at other organisms to deliver payloads to using our payload delivery device. Having screened about a dozen single-celled, freshwater eukaryotes for phagocytosis of E. coli and ability to survive in various concentrations of LB, we are now focusing our efforts on [http://en.wikipedia.org/wiki/Tetrahymena Tetrahymena], [http://en.wikipedia.org/wiki/Blepharisma Blepharisma] and [http://en.wikipedia.org/wiki/Paramecium Paramecia]. We have already determined that these three types of organisms will readily eat E. coli and are capable of surviving in mixtures of their culturing media and LB.
| Tetrahymena |  |  |  |
| Blepharisma |  |  |  |
| Paramecia |  |  |  |
