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Team:Berkeley/Project/Other organisms
From 2010.igem.org
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Our project focused on the delivery of payloads to the cytoplasms of choanoflagellates, but its simple design makes it applicable to a host of other eukaryotes. Our design is only limited in three main aspects as to its potential targets: | Our project focused on the delivery of payloads to the cytoplasms of choanoflagellates, but its simple design makes it applicable to a host of other eukaryotes. Our design is only limited in three main aspects as to its potential targets: | ||
| - | * The target organism must be able to naturally | + | * The target organism must be able to naturally phagocytose <i>Escheria Coli</i>. |
* The target organism's phagocytic vesicles must contain cholesterols susceptible to our [https://2010.igem.org/Team:Berkeley/Project/Vesicle_Buster Vesicle-Buster] device. | * The target organism's phagocytic vesicles must contain cholesterols susceptible to our [https://2010.igem.org/Team:Berkeley/Project/Vesicle_Buster Vesicle-Buster] device. | ||
* The target organism must be able to survive in media that is compatible with our [https://2010.igem.org/Team:Berkeley/Project/Self_Lysis Self-Lysis] device. | * The target organism must be able to survive in media that is compatible with our [https://2010.igem.org/Team:Berkeley/Project/Self_Lysis Self-Lysis] device. | ||
| + | We have started looking at other organisms to deliver payloads to using our payload delivery device. Having screened about a dozen single-celled, freshwater eukaryotes for phagocytosis of <i>E. coli</i> and ability to survive in various concentrations of LB, we are now focusing our efforts on [[http://en.wikipedia.org/wiki/Tetrahymena Tetrahymena]], [[http://en.wikipedia.org/wiki/Spirotrich Euplotes]] and [[http://en.wikipedia.org/wiki/Paramecium Paramecia]]. We have already determined that these three types of organisms will readily eat <i>E. coli</i> and are capable of surviving in mixtures of their culturing media and LB. | ||
| + | {| cellpadding="10" cellspacing="10" | ||
| + | |Tetrahymena | ||
| + | ||[[Image:Terta-Phase.jpg|thumb|300px|Tetrahymena: Phase contrast]] | ||
| + | ||[[Image:Tetra-Phase+GFP.jpg|thumb|300px|Tetrahymena: Phase contrast and GFP]] | ||
| + | ||[[Image:Tetra-GFP.jpg|thumb|300px|Tetrahymena: GFP only]] | ||
| + | |Euplotes | ||
| + | ||[[Image:Euplotes-Phase.jpg|thumb|300px|Euplotes: Phase contrast]] | ||
| + | ||[[Image:Euplotes-Phase+GFP.jpg|thumb|300px|Euplotes: Phase contrast and GFP]] | ||
| + | ||[[Image:Euplotes-GFP.jpg|thumb|300px|Euplotes: GFP only]] | ||
| + | |Paramecia | ||
| + | ||[[Image:Paramecia-Phase.jpg|thumb|300px|Paramecia: Phase contrast]] | ||
| + | ||[[Image:Paramecia-Phase+GFP.jpg|thumb|300px|Paramecia: Phase contrast and GFP]] | ||
| + | ||[[Image:Paramecia-GFP.jpg|thumb|300px|Paramecia: GFP only]] | ||
Revision as of 02:49, 27 October 2010
- Home
- Project
- Overview
- Motivation
- Other Organisms
- Parts
- Self-Lysis
- Vesicle-Buster
- Payload
- [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Berkeley Parts Submitted]
- Results
- Judging
- Clotho
- Human Practices
- Team Resources
- Who We Are
- Notebooks:
- [http://www.openwetware.org/wiki/Berk2010-Daniela Daniela's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Christoph Christoph's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Amy Amy's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Tahoura Tahoura's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Conor Conor's Notebook]
Our project focused on the delivery of payloads to the cytoplasms of choanoflagellates, but its simple design makes it applicable to a host of other eukaryotes. Our design is only limited in three main aspects as to its potential targets:
- The target organism must be able to naturally phagocytose Escheria Coli.
- The target organism's phagocytic vesicles must contain cholesterols susceptible to our Vesicle-Buster device.
- The target organism must be able to survive in media that is compatible with our Self-Lysis device.
We have started looking at other organisms to deliver payloads to using our payload delivery device. Having screened about a dozen single-celled, freshwater eukaryotes for phagocytosis of E. coli and ability to survive in various concentrations of LB, we are now focusing our efforts on http://en.wikipedia.org/wiki/Tetrahymena Tetrahymena, http://en.wikipedia.org/wiki/Spirotrich Euplotes and http://en.wikipedia.org/wiki/Paramecium Paramecia. We have already determined that these three types of organisms will readily eat E. coli and are capable of surviving in mixtures of their culturing media and LB.
| Tetrahymena |  |  |  | Euplotes | File:Euplotes-Phase.jpg Euplotes: Phase contrast | File:Euplotes-Phase+GFP.jpg Euplotes: Phase contrast and GFP | File:Euplotes-GFP.jpg Euplotes: GFP only | Paramecia |  |  |  |
