Team:Harvard/parts

From 2010.igem.org

(Difference between revisions)
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<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr>
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr>
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<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr>
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<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr>
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>
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<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr>
<tr><td></td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr>
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<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr>
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<tr><td></td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr>
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>
<tr><td></td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>
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<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr>
<tr><td></td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan (bacteria)/pat (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr>
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<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr>
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<tr><td></td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/b/b9/Lut2.txt">amiRNA LUT2</a></td></tr>
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>
<tr><td></td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan (bacteria)/nptII (plant)</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>

Revision as of 02:20, 27 October 2010




parts

namebiobrick nameoriginal namedescriptionresistance (bacteria)resistance (plants)sequence
V7BBa_K382000pORE Open Series Vector with BioBrick Sites (kan/pat)Agrobacterium vector open series with biobrick cloning siteKanamycinpat V7
V8BBa_K382001pORE Open Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector open series with biobrick cloning siteKanamycinnptII V8
V9BBa_K382002pORE Expression Series Vector with BioBrick Sites (kan/pat)Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat V9
V10BBa_K382003pORE Expression Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII V10
V11BBa_K382004pORE Reporter Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII V11
V12BBa_K382005pORE Reporter Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII V12
C1BBa_K382010amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownchloramphenicol-amiRNA LUT2
C2BBa_K382011amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-amiRNA BETA-OHASE 1
C3BBa_K382012amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-amiRNA LYC
A3BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructampicillin- ihpRNA Bet1
A4BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-ihpRNA LTP
A2BBa_K382017amiRNA LTP LTP knockdown constructampicillin- amiRNA LTP
A4BBa_K382018PDK intron hairpinIntron hairpin constructampicillin-link
A5BBa_K382019ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-ihpRNA GER
F4BBa_K382020BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-link
F5BBa_K382021MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-link
F1BBa_K382022pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-link
F2BBa_K382023NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-link
F3BBa_K382024NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on 5' end.chloramphenicol-link
BBa_K382025Miraculin StrepII N-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
BBa_K382026Brazzein StrepII N-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
F9BBa_K382027Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
F8BBa_K382028Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
GF5BBa_K382030RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.chloramphenicol-link
GF4BBa_K382031RXRLc Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozideampicillin-link
BBa_K382033Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide chloramphenicol-link
BBa_K382034Act2pLacOActin promoter with LacO sites ampicillin-link
GF3BBa_K382035LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-link
GF4BBa_K382031RXRLc Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozideampicillin-link
GF5BBa_K382036RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.ampicillin-link
BBa_K382037Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide ampicillin-link
F6BBa_K382040Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
F7BBa_K382041Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
BBa_K382050MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencekan (bacteria)/pat (plant)-link
BBa_K382051MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencekan (bacteria)/nptII (plant)-link
BBa_K382052BrazzeinSweet-tasting protein. 54 amino acid sequence. kan (bacteria)/pat (plant)-link
BBa_K382053BrazzeinSweet-tasting protein. 54 amino acid sequence. kan (bacteria) / nptII (plant)-link
BBa_K382054amiRNA GFPamiRNA construct for GFP knockdownkan (bacteria)/pat (plant)-link
BBa_K382055amiRNA GFPamiRNA construct for GFP knockdownkan (bacteria)/nptII (plant)-link
BBa_K382058amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownkan (bacteria)/pat (plant)-amiRNA LUT2
BBa_K382059amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownkan (bacteria)/pat (plant)-amiRNA BETA-OHASE 1
BBa_K382060amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownkan (bacteria)/pat (plant)-amiRNA LYC
BBa_K382061amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownkan (bacteria)/nptII (plant)-amiRNA LUT2
BBa_K382062amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownkan (bacteria)/nptII (plant)-amiRNA BETA-OHASE 1
BBa_K382063amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownkan (bacteria)/nptII (plant)-amiRNA LYC
BBa_K382064amiRNA LTP LTP knockdown constructkan (bacteria)/pat (plant)- amiRNA LTP
BBa_K382065amiRNA LTP LTP knockdown constructkan (bacteria)/nptII (plant)- amiRNA LTP
BBa_K382066ihpRNA Ger Intron hairpin Ger Knockdown constructkan (bacteria)/pat (plant)-ihpRNA GER
BBa_K382067ihpRNA Ger Intron hairpin Ger Knockdown constructkan (bacteria)/nptII (plant)-ihpRNA GER
BBa_K382068ihpRNA BetV1Intron hairpin Bet Knockdown constructkan (bacteria)/pat (plant)- ihpRNA Bet1
BBa_K382069ihpRNA BetV1Intron hairpin Bet Knockdown constructkan (bacteria)/nptII (plant)- ihpRNA Bet1
BBa_K382070ihpRNA LTPIntron hairpin LTP Knockdown constructkan (bacteria)/pat (plant)-ihpRNA LTP
BBa_K382071ihpRNA LTPIntron hairpin LTP Knockdown constructkan (bacteria)/nptII (plant)-ihpRNA LTP

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