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Week12 8/29/10-9/4/10
From 2010.igem.org
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Ragan left for home today and left MAGE in our hands. He asked us to test his tsqA knockouts to determine if they were in fact true knockouts. | Ragan left for home today and left MAGE in our hands. He asked us to test his tsqA knockouts to determine if they were in fact true knockouts. | ||
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Latest revision as of 02:10, 27 October 2010
Week12 Highlights
We began assembling LacP-RBS-CHS3. We defrosted our -20 and in the process reorganized the -20 and threw out unnecessary DNA.
8/30/10
Screened MAGE culture for knockouts.
8/31/10
Attempted to assemble LacP-RBS-CHS3 --> accidentally cut CHS3 with P (contains a P site)
Reorganized the racks in the -20.
Threw out unnecessary/old DNA from our -20.
Moved all of our racks from our -20 to another -20 in order to defrost our -20.
tqsA Knockouts FOUND (?) in MAGE culture upon screening.
9/1/10
Ragan left for home today and left MAGE in our hands. He asked us to test his tsqA knockouts to determine if they were in fact true knockouts.
9/2/10
Day Off
9/3/10
Reorganized -20 DNA stock. Created racks for individual teammates to stay organized once the school year starts.
