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Revision as of 02:28, 8 July 2010 (view source) Infekt (Talk | contribs) (New page: ==7/7/10== ''Jennifer and Alex - in Lin Lab'' Made CaCl2 stock solution <math>$a_{10}$</math>) ← Older edit		Revision as of 03:04, 8 July 2010 (view source) Infekt (Talk | contribs) (→7/7/10) Newer edit →
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	==7/7/10==		==7/7/10==
	''Jennifer and Alex - in Lin Lab''		''Jennifer and Alex - in Lin Lab''
-	Made CaCl2 stock solution <math>$a_{10}$</math>	+
		+	Made CaCl<sub>2</sub> stock solution: 0.1M - 50mL
		+	*molar mass = 111 g/mol
		+	*(.1 mol/L)(.05 L)(111 g/mol) = 555mg
		+	*dissolved 555 mg CaCl<sub>2</sub> in 50mL DIwater
		+	*vacuum filtered into 50mL tube
		+	Made ampicillin stock solution:
		+	-1 g ampicillin
		+	-5mL DIwater
		+	-ethanol
		+	*filtered by syringe into 15mL tube
		+	*put into ten 1mL alliquots in 1.5mL tubes
		+	*stored in -20°C in ERB lab
		+	Inoculated ''E. coli'' DH5α from frozen stock into 12 mL LB broth
		+	*put in 30°C, 200 rpm shaking, overnight

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Revision as of 03:04, 8 July 2010

7/7/10

Jennifer and Alex - in Lin Lab

Made CaCl₂ stock solution: 0.1M - 50mL

• molar mass = 111 g/mol
• (.1 mol/L)(.05 L)(111 g/mol) = 555mg
• dissolved 555 mg CaCl₂ in 50mL DIwater
• vacuum filtered into 50mL tube

Made ampicillin stock solution:

 -1 g ampicillin
 -5mL DIwater
 -ethanol

• filtered by syringe into 15mL tube
• put into ten 1mL alliquots in 1.5mL tubes
• stored in -20°C in ERB lab

Inoculated E. coli DH5α from frozen stock into 12 mL LB broth

• put in 30°C, 200 rpm shaking, overnight