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Week17 10/3/10-10/9/10
From 2010.igem.org
(New page: ===10/3/10=== Timi miniprepped the CP-LacPI-GFP cultures and digested them with E/P. She then poured a big and a small gel. Kevin came in and ran the digested CP-LacPI-GFP, digested CP-La...) |
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| + | We ran into problems while running the gels for our CP-LacPI-GFP parts. We decided to use new buffer. | ||
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===10/3/10=== | ===10/3/10=== | ||
Timi miniprepped the CP-LacPI-GFP cultures and digested them with E/P. She then poured a big and a small gel. | Timi miniprepped the CP-LacPI-GFP cultures and digested them with E/P. She then poured a big and a small gel. | ||
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===10/4/10=== | ===10/4/10=== | ||
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===10/6/10=== | ===10/6/10=== | ||
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===10/7/10=== | ===10/7/10=== | ||
Latest revision as of 01:58, 27 October 2010
Week17 Highlights
We ran into problems while running the gels for our CP-LacPI-GFP parts. We decided to use new buffer.
10/3/10
Timi miniprepped the CP-LacPI-GFP cultures and digested them with E/P. She then poured a big and a small gel.
Kevin came in and ran the digested CP-LacPI-GFP, digested CP-LacPI with digested Cp and digested LacPI parts.
10/4/10
Day Off
10/5/10
Timi and Kevin came in to discuss why their gels were running strangely. We believe there is something wrong with our buffer, so we are swapping everything out with a fresh batch of Prof. Mordacq's 0.5x TBE buffer. Timi poured a large gel that we will be using tomorrow.
10/6/10
Day Off
10/7/10
Timi- took part 2-17F out of the DNA kit. Transformed into T10 cells to grow overnight.
10/8/10
Kevin- ran gel of the CP-LacPI parts. Gel results are still inconclusive. Also put in an overnight culture of the 2-17F part into the incubator for Timi.
10/9/10
Timi- Miniprepped the 2-17F part. Digested the DNA with E/P and ligated the 2-17F GFP part into Chlor and Tet backbone.
