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Team:TU Delft/Project/sensing/results
From 2010.igem.org
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===pCaiF strength=== | ===pCaiF strength=== | ||
| - | ====BBa_K398326==== | + | ====[http://partsregistry.org/Part:BBa_K398326 BBa_K398326]==== |
pCaiF is one of our Biobricks of the sensing project. It's function is to express proteins under glucose limitation conditions. In order to measure its strength we attached the GFP generator in front of it. | pCaiF is one of our Biobricks of the sensing project. It's function is to express proteins under glucose limitation conditions. In order to measure its strength we attached the GFP generator in front of it. | ||
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| + | [[Image:TU_Delft_pCaiF_final.jpg]] | ||
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| + | [[Image:TU_Delft_pCaiF_10g.jpg]] | ||
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| + | [[Image:TU_Delft_pCaiF_5g.jpg]] | ||
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| + | [[Image:TU_Delft_pCaiF_2g.jpg]] | ||
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| + | [[Image:TU_Delft_pCaiF_diauxic.jpg]] | ||
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| + | [[Image:TU_Delft_pCaiF_LB.jpg]] | ||
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| + | [[Image:TU_Delft_pCaiF_05LB.jpg]] | ||
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Different media, and carbon source concentrations were tested in order to obtain an insight about the protein production during starvation. | Different media, and carbon source concentrations were tested in order to obtain an insight about the protein production during starvation. | ||
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Also, it is well known that this promotor it's active during diauxic shifts, that's why we measured the GFP production using glucose as a first carbon source (concentration of 1g/L) and Potassium Laurate (5mM) as a secondary carbon source. The results that we got suggest that the part works like a charm! During glucose starvation high levels of cAMP can be found in cells, which enhances the expression of the proteins downstream of this promoter. On the other hand when the secondary carbon source is being catabolized by the cells, cAMP levels drop and thus the expression of genes regulated by pCaiF. | Also, it is well known that this promotor it's active during diauxic shifts, that's why we measured the GFP production using glucose as a first carbon source (concentration of 1g/L) and Potassium Laurate (5mM) as a secondary carbon source. The results that we got suggest that the part works like a charm! During glucose starvation high levels of cAMP can be found in cells, which enhances the expression of the proteins downstream of this promoter. On the other hand when the secondary carbon source is being catabolized by the cells, cAMP levels drop and thus the expression of genes regulated by pCaiF. | ||
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View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398326 BBa_K398326 in the '''parts registry'''] | View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398326 BBa_K398326 in the '''parts registry'''] | ||
Revision as of 01:28, 27 October 2010
Sensing Results
pCaiF strength
[http://partsregistry.org/Part:BBa_K398326 BBa_K398326]
pCaiF is one of our Biobricks of the sensing project. It's function is to express proteins under glucose limitation conditions. In order to measure its strength we attached the GFP generator in front of it.
Different media, and carbon source concentrations were tested in order to obtain an insight about the protein production during starvation.
Also, it is well known that this promotor it's active during diauxic shifts, that's why we measured the GFP production using glucose as a first carbon source (concentration of 1g/L) and Potassium Laurate (5mM) as a secondary carbon source. The results that we got suggest that the part works like a charm! During glucose starvation high levels of cAMP can be found in cells, which enhances the expression of the proteins downstream of this promoter. On the other hand when the secondary carbon source is being catabolized by the cells, cAMP levels drop and thus the expression of genes regulated by pCaiF.
View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398326 BBa_K398326 in the parts registry]
Specified Components <partinfo>K398326 SpecifiedComponents</partinfo>
Designer: <partinfo>K398326 Designer</partinfo>
Status: <partinfo>K398326 Status</partinfo>
View [http://partsregistry.org/wiki/index.php?title=Part:BBa_K398331 BBa_K398331 in the parts registry]
Specified Components <partinfo>K398331 SpecifiedComponents</partinfo>
Designer: <partinfo>K398331 Designer</partinfo>
Status: <partinfo>K398331 Status</partinfo>
