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Team:Harvard/parts
From 2010.igem.org
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| - | + | <tr><td><b>name</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr> | |
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| - | + | <tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr> | |
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| - | + | <tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr> | |
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| + | <tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites (kan/pat)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr> | ||
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| + | <tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr> | ||
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| + | <tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr> | ||
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| + | <tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr> | ||
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<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr> | <tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr> | ||
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| - | + | <tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr> | |
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| + | <tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr> | ||
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| + | <tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>ampicillin</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr> | ||
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| + | <tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr> | ||
<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr> | <tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>ampicillin</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr> | ||
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<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href=</a></td></tr> | <tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href=</a></td></tr> | ||
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| - | <tr><td> | + | <tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr> |
| - | <tr><td> | + | |
| - | + | <tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr> | |
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| - | + | <tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr> | |
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| + | tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr> | ||
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| + | <tr><td>F2</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr> | ||
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| + | <tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr> | ||
| - | <tr><td></td><td> | + | <tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr> |
| - | <tr><td></td><td> | + | <tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr> |
<tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr> | <tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr> | ||
Revision as of 23:57, 26 October 2010
parts
| name | biobrick name | original name | description | resistance (bacteria) | resistance (plants) | sequence |
| V7 | BBa_K382000 | pORE Open Series Vector with BioBrick Sites (kan/pat) | Agrobacterium vector open series with biobrick cloning site | Kanamycin | pat | V7 |
| V8 | BBa_K382001 | pORE Open Series Vector with BioBrick Sites (kan/nptII) | Agrobacterium vector open series with biobrick cloning site | Kanamycin | nptII | V8 |
| V9 | BBa_K382002 | pORE Expression Series Vector with BioBrick Sites (kan/pat) | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | pat | V9 |
| V10 | BBa_K382003 | pORE Expression Series Vector with BioBrick Sites (kan/nptII) | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | nptII | V10 |
| V11 | BBa_K382004 | pORE Reporter Series Vector with BioBrick Sites (kan/nptII) | Agrobacterium vector with gusA reporter and biobrick cloning site | Kanamycin | nptII | V11 |
| V12 | BBa_K382005 | pORE Reporter Series Vector with BioBrick Sites (kan/nptII) | Agrobacterium vector with smgfp reporter and biobrick cloning site | Kanamycin | nptII | V12 |
| C1 | BBa_K382010 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown | chloramphenicol | - | amiRNA LUT2 |
| C2 | BBa_K382011 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | chloramphenicol | - | amiRNA BETA-OHASE 1 |
| C3 | BBa_K382012 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | chloramphenicol | - | amiRNA LYC |
| A3 | BBa_K382014 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | ampicillin | - | ihpRNA Bet1 |
| A4 | BBa_K382015 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | - | ihpRNA LTP |
| A2 | BBa_K382017 | amiRNA LTP | LTP knockdown construct | ampicillin | - | amiRNA LTP |
| A4 | BBa_K382018 | PDK intron hairpin | Intron hairpin construct | ampicillin | - | |
| A5 | BBa_K382019 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | - | ihpRNA GER |
| F4 | BBa_K382020 | Brazzein | Sweet-tasting protein. 54 amino acid sequence. | chloramphenicol | - | link |
| F5 | BBa_K382021 | Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | chloramphenicol | - | link | F1 | BBa_K382022 | pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. | chloramphenicol | - | link |
| F2 | BBa_K382023 | NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. | chloramphenicol | - | link |
| F3 | BBa_K382024 | NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on 5' end. | chloramphenicol | - | link |
| BBa_K382025 | Miraculin StrepII N-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link | |
| BBa_K382026 | Brazzein StrepII N-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link | |
| F9 | BBa_K382027 | Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link |
| F8 | BBa_K382028 | Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link |
| GF5 | BBa_K382030 | RXRHm | Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide. | chloramphenicol | - | link |
| GF4 | BBa_K382031 | RXRLc | Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide | ampicillin | - | link |
| BBa_K382033 | Ecdysone Receptor | Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide | chloramphenicol | - | link | |
| BBa_K382034 | Act2pLacO | Actin promoter with LacO sites | ampicillin | - | link | |
| GF3 | BBa_K382035 | LacIN | Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter | chloramphenicol | - | link |
| GF4 | BBa_K382031 | RXRLc | Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide | ampicillin | - | link |
| BBa_K382035 | LacIN | Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter | chloramphenicol | - | link | |
| GF5 | BBa_K382036 | RXRHm | Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide. | ampicillin | - | link |
| BBa_K382037 | Ecdysone Receptor | Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide | ampicillin | - | link | |
| F6 | BBa_K382040 | Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | link |
| F7 | BBa_K382041 | Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | link |
| BBa_K382050 | Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | kan (bacteria)/pat (plant) | - | link | |
| BBa_K382051 | Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | kan (bacteria)/nptII (plant) | - | link | |
| BBa_K382052 | Brazzein | Sweet-tasting protein. 54 amino acid sequence. | kan (bacteria)/pat (plant) | - | link | |
| BBa_K382053 | Brazzein | Sweet-tasting protein. 54 amino acid sequence. | kan (bacteria) / nptII (plant) | - | link | |
| BBa_K382054 | amiRNA GFP | amiRNA construct for GFP knockdown | kan (bacteria)/pat (plant) | - | amiRNA LUT2 | |
| BBa_K382055 | amiRNA GFP | amiRNA construct for GFP knockdown | kan (bacteria)/nptII (plant) | - | amiRNA LUT2 | |
| BBa_K382058 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown | kan (bacteria)/pat (plant) | - | amiRNA LUT2 | |
| BBa_K382059 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | kan (bacteria)/pat (plant) | - | amiRNA BETA-OHASE 1 | |
| BBa_K382060 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | kan (bacteria)/pat (plant) | - | amiRNA LYC | |
| BBa_K382061 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown | kan (bacteria)/nptII (plant) | - | amiRNA LUT2 | |
| BBa_K382062 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | kan (bacteria)/nptII (plant) | - | amiRNA BETA-OHASE 1 | |
| BBa_K382063 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | kan (bacteria)/nptII (plant) | - | amiRNA LYC | |
| BBa_K382064 | amiRNA LTP | LTP knockdown construct | kan (bacteria)/pat (plant) | - | amiRNA LTP | |
| BBa_K382065 | amiRNA LTP | LTP knockdown construct | kan (bacteria)/nptII (plant) | - | amiRNA LTP | |
| BBa_K382066 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | kan (bacteria)/pat (plant) | - | ihpRNA GER | |
| BBa_K382067 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | kan (bacteria)/nptII (plant) | - | ihpRNA GER | |
| BBa_K382068 | ihpRNA BetV1 | Intron hairpin Bet Knockdown construct | kan (bacteria)/pat (plant) | - | ihpRNA Bet1 | |
| BBa_K382069 | ihpRNA BetV1 | Intron hairpin Bet Knockdown construct | kan (bacteria)/nptII (plant) | - | ihpRNA Bet1 | |
| BBa_K382070 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | kan (bacteria)/pat (plant) | - | ihpRNA LTP | |
| BBa_K382071 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | kan (bacteria)/nptII (plant) | - | ihpRNA LTP |
